微流控芯片中单个细胞运输的微阀系统

Akiyuki Hasegawa, H. Uvet, K. Ohara, T. Takubo, Y. Mae, T. Arai
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引用次数: 1

摘要

近年来,人们研制出了一种微阀,用于控制微芯片通道内的各种流体。利用微流控技术在芯片内克隆体细胞是目前研究的热点。这项工作包括细胞供应、切割、分选、耦合、融合等步骤。在微流控芯片上构建了一个细胞供给系统,通过控制压力阀将单个细胞输送到目标轨道。在这里,我们制作了三种阀门,a)开通道两层阀,b)开通道三层阀,c)闭通道两层阀。我们比较了这些阀门对微通道流量的拦截条件和开启/关闭所需的时间。然后通过这些比较来评价它们是否适合细胞的顺利运输。
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Micro valve system for individual cell transportation in microfluidic chip
Recently, micro valves to control some kinds of fluids in channels in a microchip have been developed. Now we pay attention to in-chip somatic cell cloning with microfluidic technology. There are some steps, cell supply, cutting, sorting, coupling, fusion and so on, for this work. And we focused on a cell supply system spending cells one by one in microfluidic chip, and attempt to transport an individual cell to objective course by the control of pressure valves. Here, we made three kinds valves, a) open channel 2-layer valve, b) open channel 3-layer valve, and c) close channel 2-layer valve. We compare these valves about interception condition of micro channel flow and time to take for open/close. And then we evaluated them with suitable for the smooth cell transportation by these comparisons.
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