玉米(Zea mays L)抗象甲的选育

Stephen Taulu, Davis M. Lungu, Philemon H. Sohati
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引用次数: 1

摘要

进行了一项研究,其总体目标是确定玉米对象鼻虫抗性的决定因素。现场实验是在GART使用北卡罗来纳设计II进行的,有三个重复,也在Mazabuka的Nanga研究中心进行的。实验室实验在赞比亚农业研究所昆虫学实验室和赞比亚大学食品科学系进行,分别进行昆虫生物测定和生物化学试验。在昆虫生物试验中,测定了籽粒硬度、籽粒失重、中位发育期、F1代出苗率和敏感性指标的多比指数。通过生化试验测定不同基因型的蛋白质和酚类物质含量。在所有27个基因型中均存在高度显著差异。结果表明,亲本存活率占总变异的78.5%,酚含量与籽粒硬度呈显著正相关(r = 0.423***),是衡量抗性的良好指标,占总变异的10.9%。研究表明,选育抗性增强的玉米基因型是有可能的,而且易感品系的酚含量降低,但酚含量增加导致抗性增强。综上所述,酚含量和亲本存活率可作为筛选象鼻虫抗性的间接标准。贡献/独创性:本研究是为数不多的研究寄主-植物抗性发展的研究之一,因为一种害虫控制方法是安全的,对农民来说是一种方法,并且与害虫综合管理计划中的其他组成部分最兼容。
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Breeding for Weevil (Sitophilus Zeamais Motschulsky) Resistance in Maize (Zea mays L)
A study was conducted with an overall objective of establishing the determinants of weevil resistance in maize. Field experiments were done at GART using a North Carolina Design II with three replications and also at Nanga research in Mazabuka. Laboratory experiments were conducted at Zambia Agriculture Research Institute (ZARI) Entomology laboratory and University of Zambia, Food Science Department where insect bioassay and the biochemical tests were done respectively. In the insect bioassay grain hardness, grain weight loss, median development period, F1 progeny emergence and the Dobie index of susceptibility indices were measured. Protein and the Phenolic content were determined under the biochemical tests among genotypes. They were highly significant differences in all the twenty seven genotypes evaluated. Results showed that Parental survival accounted for 78.5 % of the total variation, Phenolic content was strongly and positively correlated (r = 0.423***) with grain hardness providing a good measure of resistance accounting for the 10.9 % of the total variation. The study showed the possibility of breeding maize genotypes with an increased resistance and also susceptible lines had a decreasing Phenolic content but increasing Phenolic content resulted in increased resistance. It was therefore concluded that Phenolic content and parental survival can be used as an indirect selection criteria for weevil resistance. Contribution/Originality: This study is one of very few studies which have investigated the development of the Host-plant resistance as a pest control method is safe, method to farmers and most compatible with other components in the Integrated Pest Management initiatives.
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