毛藻提取物对嗜水气单胞菌和弧菌的抑菌带试验

S. Wahyuni, M. Gazali, R. Kurniawan
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摘要

Chaetomorpha sp.是一种绿藻,含有抗菌化合物,在某些时候发现非常多。本研究的目的是观察Chaetomorpha sp.乙醇提取物对A. hydroilla和Vibrio sp.的抑制带。本研究于2022年3月完成。在廖内省大学渔业与海洋学院生物技术实验室对毛藻的提取及抗菌效果进行了测试。使用的方法是描述性方法。采用Kirby Bauer圆盘法进行敏感性试验。为了减少错误率,重复了三次。使用的大藻提取物剂量为100% (10,000 ppm)、90% (9,000 ppm)、80% (8,000 ppm)、70% (7,000 ppm)、60% (6,000 ppm)、50% (5000 ppm)、40% (4000 ppm)、30% (3000 ppm)、20% (2000 ppm)、10% (1000 ppm)和对照(土霉素)。通过观察发现,毛藻乙醇提取物在13.5 ~ 14.5 mm范围内对嗜水芽胞杆菌和弧菌的生长有较强的抑制作用,最大抑制剂量为10000 ppm,最小抑制剂量为5000 ppm,为中等抑制作用。
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Inhibition Zone Test of Chaetomorpha sp. Extract Against Aeromonas hydrophilla and Vibrio sp. Bacteria
Chaetomorpha sp. is a green algae that has anti-bacterial compounds and at certain times found very much. The purpose of this study was to see the inhibition zone produced from the ethanol extract of Chaetomorpha sp. against A. hydrophilla and Vibrio sp. This research was done in March 2022. Extraction of Chaetomorpha sp., antibacterial effectiveness testing was done at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Riau University. The method used is descriptive method. Sensitivity test was performed using the Kirby Bauer disc method. To reduce the error rate, it was repeated three times. The doses of macroalgae extract used were, doses of 100% (10,000 ppm), 90% (9,000 ppm), 80% (8,000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40 % (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline). Based on the observations, it was found that the use of ethanol extract of Chaetomorpha sp. The result is able to inhibit the growth of bacteria A. hydrophilla and Vibrio sp. at the highest dose (10000 ppm) in an range of 13.5 -14.5 mm which was classified as strong category, the lowest dose was at 5000 ppm as moderate category.
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