发酵和未发酵红薯粉的理化和微生物特性

Emmanuel Oboh
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摘要

基于本地作物面粉的理化和微生物特性,寻找合适的小麦粉替代品是本研究的重点。从尼日利亚当地市场购买黄甘薯块茎,加工成发酵和未发酵的甘薯粉。使用常规分析程序对其理化性质(近似性质和功能性质)和微生物性质进行了分析。结果表明,发酵后的红薯粉pH值从0 h时的8.8降至72 h时的5.0,蛋白质和水分近似成分得到改善,吸附水和吸油能力等功能属性得到增强。发酵菌的活菌总数为7.5 ~ 10.6 Log10 cfu/g,真菌的活菌总数为0 ~ 3.9 Log10 cfu/g。检出细菌6属,分别为芽孢杆菌属、克雷伯氏菌属、微球菌属、葡萄球菌属、乳球菌属和肠杆菌属,其中以芽孢杆菌属最占优势(33.3%),真菌属5属;72 h发酵结束时还分离出曲霉、青霉、毛霉、念珠菌和酵母菌,以曲霉属为优势菌属(44.44%)。从本研究中分离出的微生物通常与植物、环境和发酵过程有关。本研究表明,发酵有助于改善黄肉甘薯的营养成分,直接食用或加工成面粉可改善消费者的健康状况。
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PHYSIOCHEMICAL AND MICROBIOLOGICAL PROPERTIES OF FERMENTED AND UNFERMENTED SWEET POTATO FLOUR
The need for suitable wheat flour substitute from indigenous crop flours based on their physiochemical and microbiological properties is the focus of this research. Raw yellow sweet potato tubers were purchased from local Nigerian market and processed into fermented and unfermented sweet potato flours. Analysis for their physiochemical properties (proximate and functional properties) and microbiological properties were executed using customary analytical routines. Findings revealed that fermenting sweet potato flour decreased the pH from 8.8 at 0 hr to 5.0 at 72 hrs, improved protein and moisture proximate components and enhanced some functional attributes like water adsorption and oil adsorption capacities over their unfermented counterpart. The total viable bacteria count for the fermenting bacteria ranged from 7.5 – 10.6 Log10 cfu/g, while fungi count ranged from 0 – 3.9 Log10 cfu/g. Six (6) genera of bacteria, Bacillus, Klebsiella, Micrococcus, Staphylococcus, Lactococcus and Enterobacter were isolated with Bacillus spp being the most dominant genera (33.3%), while five (5) Fungi genera; Aspergillus, Penicillium, Mucor, Candida and Saccharomyces were also isolated at the end of the 72 hrs fermentation with Aspergillus spp., the most dominant genera (44.44%). Microbes isolated from this study are generally associated with plant, environment and fermentation processes. This study shows that fermentation helps to improve nutritive composition of yellow fleshed sweet potato which when directly consumed or processed into flours would improve the health status of consumers.
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