脱水介质和温度对脱水时间和组织收缩率的影响

Ma Brown, R. Reed, R. Henry
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引用次数: 44

摘要

为了评估目前流行的塑化脱水技术的效果,我们将多种器官脱水到:1。2.确定浸渍试样彻底脱水所需的最短时间。测量脱水时的组织收缩。对塑化常用的脱水剂(常温、低温丙酮和常温甲醇)进行了评价。冷丙酮脱水产生的组织收缩最小。收缩是最大的分级甲醇系列。对于低温和室温丙酮脱水,丙酮脱水所需的最短时间为5天。
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Effects of Dehydration Mediums and Temperature on Total Dehydration Time and Tissue Shrinkage
To assess the efficacy of current popular plastination dehydration techniques, a variety of organs were dehydrated to: 1. Determine the minimal length of time necessary to thoroughly dehydrate specimens for impregnation and 2. Measure tissue shrinkage during dehydration. Dehydrating agents commonly used for plastination (both room and cold temperature acetone and room temperature methanol) were evaluated. Cold acetone dehydration produced the least amount of tissue shrinkage. Shrinkage was greatest in the graded methanol series. Minimal length of time necessary for acetone dehydration was five days for both cold and room-temperature acetone dehydration.
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