集成磁力搅拌泵的双室微生物反应器测量Caco-2细胞的跨膜转运

H. Kimura, H. Sakai, S. Ostrovidov, T. Yamamoto, Y. Sakai, T. Fujii
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引用次数: 0

摘要

我们已经开发并实验展示了一种嵌入搅拌型微泵的微生物反应器,用于片上培养基的灌注,以及用于片上和实时荧光检测的光纤连接器。用聚酯半透膜将细胞培养室分成两室,实现细胞培养条件的极化和两室间荧光运动的检测。以小肠Caco-2模型细胞p -糖蛋白活性测定罗丹明-123的运动作为评价微生物反应器的指标。结果,成功检测了罗丹明-123从Caco-2细胞基底外侧到根尖侧的荧光强度变化
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Two-Compartments Microbioreactor with Integrated Magnetic Stirrer Pump for Measurement of Transmembrane Transport of Caco-2 Cells
We have developed and experimentally demonstrated a microbioreactor embedded with stirrer-type micropumps for on-chip perfusion of culture medium and optical fiber connectors for on-chip and real-time fluorescent detection. The cell culture chamber was separated two compartments by a polyester-made semi-permeable membrane, which can realize polarized cell culture condition and detection of fluorescent movement in-between the two-compartments. As an evaluation of the microbioreactor, it was measured that Rhodamine-123 movement by the activity of P-glycoprotein of a small intestine model cell of Caco-2. As a result, the change of fluorescent intensity of Rhodamine-123 from basolateral side to apical side of Caco-2 cells was successfully detected
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