昆虫病原真菌球孢白僵菌(Beauveria bassiana, Balsamo)大规模生产基质的评价

R. Singh, V. Singh, Ajay Kumar, Joginder Singh, Ankit Kumar, Ravi Kumar
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摘要

本研究题为“液体和固体培养基上昆虫病原真菌球孢白杆菌(Metsch.)的大规模生产”,于2018- 2019年在密鲁特萨达尔瓦拉布海帕特尔农业科技大学昆虫学系生物防治实验室进行。总共有8个处理,包括对照,在随机区组设计中重复了3次。对研究结果进行了总结。不同底物对球孢白僵菌孢子/ml量产的影响显著较高。2018-19年间,在T10味葡萄糖肉汤(SDB)中接种不同处理后第7、14、21、28、35天记录的数据显示,T10味葡萄糖肉汤(SDB)在液体培养基中使球孢白弧菌的产量降至49.48个孢子/ml。孢子产生的其他治疗顺序与T11马铃薯葡萄糖肉汤(PDB)(38.52),在治疗和液体培养基和固体培养基T8豇豆豇豆属unguiculata +你们糖浆+ 1% + 1.0克葡萄糖(28.73),其次是与T7黑克豇豆属你们蒙戈+糖浆+ 1% + 1.0克葡萄糖(27.52孢子/ ml, T5豇豆豇豆属unguiculata +糖浆+ 1%(17.53孢子/毫升),T4黑克豇豆属蒙戈+糖浆+ 1%(14.87孢子/毫升),T2豇豆花蜜+糖蜜(10.50孢子/ml)、T1黑克花蜜+糖蜜(8.35孢子/ml)、T9克花蜜+糖蜜+ 1%叶叶+ 1.0 g葡萄糖(3.92孢子/ml)、T6克花蜜+糖蜜+ 1%叶叶(2.85孢子/ml)和T3克花蜜+糖蜜。
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Evaluate on substrates for mass production of entomopathogenic fungus Beauveria bassiana (Balsamo)
The present investigation entitled “Mass Production of entomopathogenic fungai Beauveria bassiana (Metsch.) on liquid and solid media” was carried out at Bio Control Laboratory, Department of Entomology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut during 2018-19. In all there were eight treatments including control replicated thrice in a randomized block design. The results obtained in the study are summarized. The effect of different substrates for the mass production of Beauveria bassiana spore/ml was significantly higher recorded. Data recorded on 7, 14, 21, 28, 35 after the day after inoculation of various treatments in the, T10 Savoured dextrose broth (SDB), was the best treatment by bringing down the B. Bassiana production up to (49.48) spore/ml in liquid medium during the 2018-19 years. The other treatments in order of spore producing was with T11 Potato dextrose broth (PDB) (38.52), in liquid medium and solid medium in the treatment and T8 Cowpea Vigna unguiculata + Molasses + 1% YE + 1.0 g Dextrose (28.73), followed by with T7 Black gram Vigna mungo + Molasses + 1% YE + 1.0 g Dextrose (27.52 spore/ml, T5 Cowpea Vigna unguiculata + Molasses + 1% YE (17.53 spore/ml), T4 Black gram Vigna mungo + Molasses + 1% YE (14.87 spore/ml), T2 Cowpea Vigna unguiculata + Molasses (10.50 spore/ml), T1 Black gram Vigna mungo + Molasses (8.35 spore/ml), T9 Gram Cicer aeritinum + Molasses + 1% YE + 1.0 g Dextrose (3.92 spore/ml), T6 Gram Cicer aeritinum + Molasses + 1% YE (2.85 spore/ml) and T3 Gram Cicer aeritinum + Molasses respectively.
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