hoechst33258与bsa配合物的光谱特性

A. Antonyan, A. Karapetyan, N. Petrosyan, P. Vardevanyan
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摘要

采用吸收光谱法、差示光谱法和荧光光谱法研究了dna特异性化合物Hoechst 33258与牛血清白蛋白(BSA)的相互作用。根据该配体与BSA配合物的吸收光谱和荧光光谱,在r (r=配体/白蛋白)变化的宽间隔内,构建了H33258-BSA配合物的吸收(A/A0)和荧光(F/F0)最大值相对变化与大分子浓度的依赖曲线。由于络合物的形成,A/A0的依赖曲线随着蛋白质浓度的增加而单调降低。结果表明,F/F0的依赖曲线不是线性的,它由两个区域组成:在蛋白质浓度较低时,该曲线线性下降,在蛋白质浓度较高浓度时,该曲线通过最小点开始线性上升。H33258-BSA配合物的微分光谱具有低、高显色效应和低显色位移的特征:低浓度时出现低显色效应,高浓度时(从配体/白蛋白的一定比例开始),微分光谱出现低显色位移和高显色效应。所获得的数据发现Hoechst 33258是dna特异性配体,与白蛋白紧密结合。
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SPECTRAL CHARACTERISTICS OF THE COMPLEXES OF HOECHST33258 WITH BSA
Study on the interaction of DNA-specific compound Hoechst 33258 with bovine serum albumin (BSA) has been carried out, using the methods of absorption, differential and fluorescence spectroscopies. On the basis of the absorption and fluorescence spectra of the complexes of this ligand with BSA, in wide interval of the change of r (r=ligand/albumin), the dependence curves of the relative changes of the absorption (A/A0) and fluorescence (F/F0) maxima of the complexes H33258-BSA on the macromolecule concentration, were constructed. The dependence curve of A/A0 monotonously decreases along with protein concentration increasing due to the formation of the complexes. It was revealed that the dependence curve of F/F0 is not linear and is consisted of two regions: at low concentrations of the protein this curve decreases linearly, and passing through the minimum point, at relatively high concentrations of the protein starts linearly increasing. It was also shown that the differential spectra of the complexes H33258-BSA are characterized by hypo- and hyperchromic effects and hypsochromic shift: at low concentrations of the protein a hypochromic effect occurs, at higher concentrations of the protein (beginning from the certain ratios ligand/albumin ~1:1), the differential spectra are hypsochromically shifted with hyperchromic effect. The obtained data find out that Hoechst 33258, being DNA-specific ligand, tightly binds to albumin.
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