特定神经元回路中突触谷氨酸瞬态的荧光成像

Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN) Pub Date : 1900-01-01 DOI:10.1364/omp.2023.otu3e.4

Ruqiang Liang, Junqing Sun, Ryan G. Natan, Boyu Lyu, Guoqiang Yu, Yihan Jin, Hwai-Jong Cheng, K. Zito, Na Ji, Lin Tian

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引用次数: 0

摘要

设计了一种可分裂的谷氨酸基因编码强度荧光传感器。它可以在突触间隙中重建，并报告当非荧光半部分靶向突触前和突触后神经元时的谷氨酸瞬态。

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Fluorescent imaging of synaptic glutamate transients in defined neuronal circuits

A splittable genetically encoded intensimetric fluorescent sensor for glutamate is engineered. It can reconstitute across synaptic clefts and report glutamate transients when the nonfluorescent halves are targeted to pre- and post-synaptic neurons in a circuit.

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Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)

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