Extraction and quantification of acrylic acid from acrylamidase-catalyzed reaction produced by Bacillus tequilensis

The reduction of non-renewable material has raised significant concerns for several years over the sustainable production of organic acids through bio-based methods in the world. One such way to overcome this problem is to use reactive extractants, in which appropriate extractants are employed to recover various organic and inorganic acids. The extraction of acrylic acid by solvent extraction is an illustration of this technique. The current study focuses on the synthesis of acrylic acid from acrylamidase produced by Bacillus tequilensis , succeeded by acid extraction from the amidase-catalyzed reaction by a solvent technique. Among the various solvents, ethyl acetate (2:1, v/v) was established as the most appropriate solvent for the extraction. Exactly 65 mg of raw acrylic acid was recovered from 20 ml of the amidase-catalyzed reaction. Various analytical methods such as thin layer chromatography, fourier transform infrared spectroscopy, high-performance liquid chromatography (HPLC), and mass spectrophotometry were accomplished for the identification, validation, and quantification of the extracted acrylic acid. The m / z value of acrylic acid obtained in the extracted product was 73.18, which was similar to the standard acrylic acid. From HPLC, almost 34% of bioconversion was quantified (3.4 mM) from 10 mM of acrylamide consumed. The extracted acrylic acid can be further exploited as chemical intermediates and pharmaceuticals in the future.