组织培养基中添加活性炭可提高大豆基因型依赖性生物量产量

D. A. Barbosa, E. G. G. Barbosa, M. D. C. Molinari, Renata Fuganti Pagliarini, S. R. Marin, Daniel Rockenbach Marin, L. M. Mertz-Henning, A. Nepomuceno
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引用次数: 2

摘要

由于其在全球农业综合经营模式中的重要作用,大豆积极地带动了生产国的国民经济。然而,由害虫和气候变化引起的生物和非生物因素分别可以破坏其生产力,从而破坏商业市场。因此,培育对这些负面环境因素更耐受的植物一直是科学研究的目标之一。在获得基因改良植物的管道中,组织培养方案通常是一个瓶颈,因为这个阶段的效率可能依赖于基因型。因此,本研究的目的是评估两种大豆基因型(BRS 283和BRS 537)在4种不同基质(蛭石、沙子、含活性炭的培养基和对照MS培养基和葡萄糖)上的根再生过程。以根系长度(cm²)、干质量(mg)、体积(mm³)、表面积(mm²)和直径(mm)测定根系发育情况。结果表明,在活性炭培养基中,两种基因型大豆的根均比蛭石和砂土培养基长得更长,根系干质量更高,根系长度也更长。我们得出结论,组织培养的效率是基因型依赖的,因为所检测的基因型表现出显著不同的表型反应。在组织培养基中添加活性炭可促进两种基因型的根系生长。因此,这种培养基很可能也可以成功地应用于其他大豆基因型,或其他具有类似组织培养程序的作物,以促进在进一步发育阶段更好的生根和植株建立。
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Activated charcoal added to tissue culture media increases genotype-dependent biomass production in soybean
Due to its important participation in the agribusiness model worldwide, soybean actively drives national economies in producing countries. However, biotic and abiotic factors caused by pests and climate changes, respectively, can disrupt its productivity and consequently the business market. For this reason, the development of plants more tolerant to these negative environmental elements has been frequently one of the goals of scientific research. In the pipeline to obtain genetically improved plants, tissue culture protocols often represent a bottleneck, since the efficiency at this stage can be genotype-dependent. Therefore, the objective of this work was to evaluate the root regeneration process of two soybean genotypes (BRS 283 and BRS 537) in four different substrates (vermiculite, sand, medium containing activated charcoal and, control – MS medium and glucose). The rooting development was measured by the root’s length (cm²), dry mass (mg), volume (mm³), surface area (mm²), and diameter (mm). Results showed that in the activated charcoal medium, for both soybean genotypes, roots grew longer and presented a higher dry mass of roots, and root length when compared to vermiculite and sand substrates. We concluded that the efficiency of tissue culture is genotype-dependent since assayed genotypes presented phenotypical responses significantly different. The supplementation of tissue culture medium with active charcoal improved root growth for both genotypes. Therefore, it is likely that this medium can be also successfully applied to other soybean genotypes, or to other crops with similar tissue culture procedures to promote better rooting and plant establishment in further developmental stages.
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