巨藻和小球藻甲醇和乙醇提取物DPPH抗自由基活性及总酚含量的研究

D. Dimova, D. Dobreva, V. Panayotova, L. Makedonski
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引用次数: 3

摘要

藻类是一种广泛使用的膳食补充剂。此外,它们可以是抗氧化剂(色素、生物碱、类胡萝卜素、酚酸、硫酸多糖和长链多不饱和脂肪酸等)的重要来源,可以代替合成的。藻类的不同营养成分取决于类别、种类、栖息地、成熟度和环境条件。目的:研究黑海大藻硬藻和小球藻抗氧化活性(AOA)和总酚含量(TPC)的差异。此外,所获得的结果将显示它们作为抗氧化剂的天然来源的潜力。材料与方法:以海洋大藻刚性Ulva和微藻小球藻为溶剂提取液,测定其抗自由基活性和总酚含量。结果与讨论:所有分析的提取物(甲醇和乙醇)DPPH试验和TPC均呈阳性。微藻小球藻和大藻刚性Ulva的甲醇提取物对废自由基的清除作用均优于同种植物材料的乙醇提取物。结果表明,这两种藻类作为抗氧化剂的天然来源可能是由于酚类和其他具有抗氧化活性的化合物的含量。结论:刚性藻和小球藻均可作为抗氧化剂和酚酸的来源,可添加到新型功能食品和保健品中,也可作为医药和化妆品的基础。
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DPPH antiradical activity and total phenolic content of methanol and ethanol extracts from macroalgae (Ulva rigida) and microalgae (Chlorella)
Introduction: Algae are widely popular as dietary supplement. Furthermore, they can be a great source of antioxidants (pigments, alkaloids, carotenoids, phenolic acids, sulfated polysaccharides and long-chain polyunsaturated fatty acids etc.) and can be used instead of synthetic ones. The different nutrient compositions of algae depend on class, species, habitats, maturity, and environmental conditions. Aim: The present study aims to investigate the differences in the antioxidant activity (AOA) and total phenolic content (TPC) of macroalgae Ulva rigida from the Black Sea and microalgae Chlorella . In addition, the obtained results will show their potential as natural sources of antioxidants. Materials and Methods: The marine macroalgae Ulva rigida and the microalgae Chlorella were used to perform different solvent extracts, which were analyzed for antiradical activity and total phenol content. Results and Discussion: All analyzed extracts (methanol and ethanol) showed positive results of the DPPH test and TPC. Both methanol extracts of microalgae Chlorella and macroalgae Ulva rigida had higher scavenging effect on used radicals for antioxidant activity compared to both ethanol extracts of the same plant material. The results show high potential as natural source of antioxidants of both algae species due perhaps to the phenolic content and other compounds having antioxidant activity. Conclusion: Both Ulva rigida and Chlorella can be used as a source of antioxidants and phenolic acids, which can be added to new functional foods and supplements, as well as be the basis of pharmaceutical and cosmetic products.
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