用自动尿液分析仪评价印度尼西亚成人尿液沉积物的参考价值

Eirene Jaquelene Tomatala, Anton Sumarpo, Hani Susanti
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引用次数: 0

摘要

需要当地建立的临床实验室参考值来解释实验室检查结果,用于筛查,诊断,预后,确定复检标准或作为显微镜确认。本研究的目的是通过使用自动尿液流式细胞仪,研究印度尼西亚人群的红细胞、白细胞、上皮细胞、上皮细胞类型、细菌、铸型、病理铸型、晶体、酵母、精子和粘液,建立尿液沉积物的参考值。2018年10月至2019年4月,在雅加达R. Said Sukanto国家警察医院进行了一项横断面研究。该研究涉及240名参与者,包括临床健康的120名男性和120名女性,年龄在18-65岁之间,尿化学、血液学、血糖、肝功能(谷丙转氨酶、谷丙转氨酶)和肾功能(尿素、肌酐)正常。参考值报告单位为微升(μL)或高性能场(HPF)或低性能场(LPF)。采用MedCalc软件Mann-Whitney检验,p值<0.05。本研究发现,男性和女性在红细胞、上皮细胞、上皮细胞种类、细菌、病理铸型和酵母菌等方面存在显著差异,其中女性患者明显较高。然而,黏液中的LPF水平在男性患者中高于女性,与目前的参考值不同。因此,两组在这些方面的参考值结果是分开的,而其他尿沉渣特征的研究结果是合并的。由于获得的大多数参考值仍在现有参考值的范围内,因此使用Sysmex UF-4000建立的参考值可能有用。
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Reference Value Evaluation of Urine Sediment in Indonesian Adult Population Using Automated Urine Analyzer
Locally established clinical laboratory reference value are required to interpret laboratory test results for screening, diagnosis, prognosis, determining retest criteria or as microscopic confirmation. The objective of this study is to establish urine sediments reference value by using automated urine flow cytometry, investigating erythrocyte, leukocyte, epithelial cells, types of epithelial cells, bacteria, casts, pathologic casts, crystals, yeast, sperm, and mucus in Indonesian population using Sysmex UF-4000. A cross sectional study was conducted in October 2018 – April 2019 at R. Said Sukanto National Police Hospital in Jakarta. The study involved 240 participants comprised of clinically healthy 120 males and 120 females aged 18-65, with normal urine chemistry, hematology, blood glucose, liver function (AST, ALT), and renal function (urea, creatinine). The reference value was reported in microliter (μL) or in High Performance Field (HPF) or Low Performance Field (LPF). Mann-Whitney test through MedCalc software was used to test significant differences with p value <0.05. This study observed significant differences between males and females in erythrocyte, epithelial cells, epithelial cell variety, bacteria, pathologic casts, and yeast”which are evidently higher in female patients. However, the mucus in LPF levels is found to be higher in male patients instead of the female, disparate than current reference value. Therefore, the reference value results were separated between the two groups in those aspects, whereas the results of other urine sediment characteristics studied were combined. As most reference value obtained are still within range of existing references, reference value established with Sysmex UF-4000 can be useful.
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