Rohmi Rohmi, Maruni Wiwin Diarti, Yunan Jiwintarum
{"title":"转基因媒介凤尾鱼、海藻、紫山药可以加速杆菌杆菌的生长","authors":"Rohmi Rohmi, Maruni Wiwin Diarti, Yunan Jiwintarum","doi":"10.33490/jkm.v7i1.312","DOIUrl":null,"url":null,"abstract":"Tuberculosis (TB) is one of the infectious diseases which is still a health problem in Indonesia caused by M. Tuberculosis. The common growth medium used to growth M. Tuberculosis is Lowenstein-Jensen. Examination by culture method has several disadvantages: the incubation period is too long to calculate the growth of M. tuberculosis that reaches 8 weeks to be able to declare negative results. LJ medium is relatively expensive and requires special manufacture that requires precision. Objective to know the result of growth rate and growth rate of M. tuberculosis from combination formulation media of local food of sea fish meal (Stolephorus sp), Purple Sweet Potato flour (Ipomoea batatas) and Euchema Spinosum Seaweed flour called TURLM media (Grass Sweet Teri Sea Medium). The method of this research is this research is an exploratory research in laboratory, using three forms of TURLM media formulation and Loweinstein-Jensen media comparator, data collected in the form of growth speed and fertility rate of M. tuberculosis, descriptive data analysis. The results showed that TRULM media formulas 1,2 and 3 can show the growth of M. Tuberculosis coloccus of ATCC 25177, with the best growth rate in TURLM Formula 3 medium which is similar to LJ control media. Fertility growth rate of M. Tuberculosis ATCC 25177 TURLM medium The best formula is TRULM Formula 3 whose fertility rate is equal to growth in Loweinstein-Jensen (LJ) control media. The conclusion of this research is that TURLM formula 3 media can be used as growth media of M. tuberculosis which can accelerate the growth of M. 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引用次数: 1
摘要
结核病(TB)是由结核分枝杆菌引起的传染病之一,在印度尼西亚仍然是一个健康问题。用于培养结核分枝杆菌的常见培养基是洛温斯坦-詹森培养基。培养法检查有几个缺点:潜伏期太长,无法计算结核分枝杆菌的生长,达到8周才能宣布阴性结果。LJ介质相对昂贵,需要精密的特殊制造。客观了解结核分枝杆菌的增长速度和增长速度的结果制定媒体组合的当地食物的海洋鱼粉(Stolephorus sp),紫地瓜粉(番薯甘薯)和棘Euchema海藻粉叫TURLM媒体(草香泰瑞海中)。本研究的方法是本研究为实验室探索性研究,采用三种形式的TURLM培养基配方和lowestein - jensen培养基比较器,以结核分枝杆菌生长速度和生育率的形式收集数据,描述性数据分析。结果表明,TURLM配方1、2、3均能显示ATCC 25177结核分枝杆菌的结肠炎生长,其中TURLM配方3培养基的生长速度最好,与LJ对照培养基相似。结核分枝杆菌ATCC 25177 TURLM培养基的育性生长率最佳配方为TRULM formula 3,其育性生长率与LJ对照培养基的育性生长率相等。本研究的结论是TURLM配方3培养基可以作为结核分枝杆菌的生长培养基,可以促进结核分枝杆菌的生长,并具有良好的生育效果。
Media Modifikasi Tepung Ikan Teri, Rumput Laut, Ubi Jalar Ungu dapat Mempercepat Pertumbuhan Mycobacterium Tuberculosis
Tuberculosis (TB) is one of the infectious diseases which is still a health problem in Indonesia caused by M. Tuberculosis. The common growth medium used to growth M. Tuberculosis is Lowenstein-Jensen. Examination by culture method has several disadvantages: the incubation period is too long to calculate the growth of M. tuberculosis that reaches 8 weeks to be able to declare negative results. LJ medium is relatively expensive and requires special manufacture that requires precision. Objective to know the result of growth rate and growth rate of M. tuberculosis from combination formulation media of local food of sea fish meal (Stolephorus sp), Purple Sweet Potato flour (Ipomoea batatas) and Euchema Spinosum Seaweed flour called TURLM media (Grass Sweet Teri Sea Medium). The method of this research is this research is an exploratory research in laboratory, using three forms of TURLM media formulation and Loweinstein-Jensen media comparator, data collected in the form of growth speed and fertility rate of M. tuberculosis, descriptive data analysis. The results showed that TRULM media formulas 1,2 and 3 can show the growth of M. Tuberculosis coloccus of ATCC 25177, with the best growth rate in TURLM Formula 3 medium which is similar to LJ control media. Fertility growth rate of M. Tuberculosis ATCC 25177 TURLM medium The best formula is TRULM Formula 3 whose fertility rate is equal to growth in Loweinstein-Jensen (LJ) control media. The conclusion of this research is that TURLM formula 3 media can be used as growth media of M. tuberculosis which can accelerate the growth of M. Tuberculosis and show good fertility rate.