猛禽

Lawrence Booth’s Book of Visions Pub Date : 2017-12-31 DOI:10.12987/9780300138184-034

F. Amat, F. Moussavi, L. Comolli, G. Elidan, K. Downing

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引用次数: 0

摘要

稳健对准和投影估计的层析重建(RAPTOR)是一个免费的软件，对准从电子显微镜获得的原始堆栈用于层析目的。它的目的是自动获得一个全精度校准可比的一个获得与延长的人工干预。对准需要图像中的基准粒子。它使用概率框架来处理非常嘈杂的图像，比如厚厚的低温EM数据集。更多的信息可以在[1]中找到。主要的想法是自动校准，所以用户不必花太多时间选择金颗粒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Raptor

Robust Alignment and Projection Estimation for Tomographic Reconstruction (RAPTOR) is a free available software to align raw stacks obtained from electron microscopes for tomographic purposes. It is intended to automatically obtain a full-precision alignment comparable to the one obtained with extended manual intervention. Fiducial particles are needed in the image for the alignment. It uses a probabilistic framework to deal with very noisy images like in thick cryo EM datasets. More information can be find in [1]. The main idea is to automate alignment, so users don’t have to spend too much time selecting gold particles.

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