大鼠内侧颗粒皮质的丘脑皮层通路:双标记光和电镜研究

Sharleen T Sakai, Kathy Bruce
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引用次数: 10

摘要

本研究的目的是确定大鼠的内侧颗粒皮质(AGm)是否被认为包含辅助运动区,并接受苍白球丘脑皮质通路。采用双标记模式,将顺行示踪剂Phaseolus vulgaris-白细胞凝集素(PHA-L)注射到大鼠髓内核(EN),同时将逆行示踪剂霍乱毒素亚单位B注射到大鼠髓内核的吻侧或尾侧。我们发现,在丘脑腹侧前-腹侧外侧核复合体(VAL)和腹侧内侧核(VM)内,丘脑半球投射呈新月形弧形。预计LD/LP, MD, Pf和CL的额外输出。LHb也有致密的突出物。此外,我们还发现从吻侧丘脑延伸出的大量细胞包括VAL和VM,通过尾侧丘脑包括MD、Po、LD/LP、Pf和CL投射到AGm的吻侧或尾侧部位。在VAL和VM中发现了最大的吻合,在与CTB标记的细胞密切相关的地方发现了许多PHA-L变异。在电镜下证实了VAL丘脑皮质神经元与苍白叶终末之间的突触接触。这些结果首次证实了苍白球丘脑皮质通路通过VAL到达大鼠的AGm。
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Pallidothalamocortical pathway to the medial agranular cortex in the rat: a double labeling light and electron microscopic study

The goal of the present study was to determine if the medial agranular cortex (AGm) that is thought to contain the supplementary motor area in the rat receives the pallidothalamocortical pathway. A double labeling paradigm was employed whereby injections of the anterograde tracer Phaseolus vulgaris-leucoagglutinin (PHA-L) into the entopeduncular nucleus (EN) were combined with injections of the retrograde tracer cholera toxin subunit B into either the rostral or caudal part AGm in the rat. We found that the pallidothalamic projections occupied a crescent shaped arc within the thalamic ventral anterior-ventral lateral nuclear complex (VAL) and ventral medial nucleus (VM). Additional efferents projected to LD/LP, MD, Pf and CL. Dense projections were also found to LHb. In addition, we found that a wide band of cells extending from rostral thalamus including VAL and VM through caudal thalamus including MD, Po, LD/LP, Pf and CL projected to either the rostral or caudal parts of the AGm. The greatest coincidence of the pallidothalamic efferents and AGm thalamocortical cells was found in VAL and VM where numerous PHA-L varicosities were found in close apposition to CTB labeled cells. Synaptic contacts between VAL thalamocortical neurons and pallidal terminals were verified in the electron microscope. These results demonstrate for the first time the pallidothalamocortical pathway by way of VAL to the AGm in the rat.

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