研制动物人工胃肠道模型的经验

A. Lukyanov, D. Donskoy, M. Vernezi, M. Mazanko, S. Studennikova
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引用次数: 0

摘要

为了“体外”实验研究益生菌对家禽和家畜微生物群发育的影响,建立了一系列类似“SHIME”的人工胃肠道模型,其形式是带有微控制器控制和监测系统的微型生物反应器复合物。在益生元对肠道微生物群发育影响的实验研究中,操作该设备的经验使其具有许多特点和局限性,本文将介绍这些特点和局限性。考虑了蠕动泵、磁力混合器的设置特点和连接管结构的选择。
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EXPERIENCE IN DEVELOPING MODELS OF ARTIFICIAL GASTROINTESTINAL TRACTS OF ANIMALS
For „in-vitro“ experimental study of the effect of probiotics on the development of microbiota of poultry and domestic animals, a line of „SHIME“ – like models of artificial gastrointestinal tracts in the form of complexes of miniature bioreactors with microcontroller control and monitoring systems was created. Experience in operating this equipment in an experimental study of the effect of prebiotics on the development of intestinal microbiota has allowed to establish a number of features and limitations, which are brought in this paper. The peculiarities of setting up peristaltic pumps, magnetic mixers and the choice of connecting tube configuration are considered.
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