放线菌素D诱导后对大鼠肝脏中镉硫蛋白和铜螯合蛋白浓度的影响

Frances A. Day, Barbara J. Coles, Frank O. Brady
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引用次数: 18

摘要

单次腹腔注射金属盐,观察铜对铜螯合蛋白的诱导作用,镉对镉硫蛋白的诱导作用,铜、镉、锌对锌硫蛋白的诱导作用。为了避免毒性作用,使用了低剂量的诱导金属,即每公斤体重5毫克锌、0.5毫克铜和0.25毫克镉。观察到的诱导高峰时间和衰减半衰期分别为:铜螯合素(9 h, 8.6 h)、镉硫蛋白(18 h, 6.80 d)、锌硫蛋白(锌大鼠,18 h, 10.1 h;铜大鼠,9 h, 18.2 h;镉大鼠,24小时,4.53天)。在诱导各种蛋白质的高峰时间给予放线菌素D(每公斤体重1毫克),在24小时后观察到螯合素或镉硫蛋白的浓度没有影响,但在锌、铜或镉诱导的锌硫蛋白的情况下,在给药后23小时内观察到浓度升高。这种行为让人想起以前在其他蛋白质和酶上看到的超诱导。我们假设肝脏中游离锌的细胞内浓度在锌硫蛋白的诱导中起着至关重要的作用,而这可能被外源性铜或镉干扰,从而诱导锌硫蛋白的合成。
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Postinductive actinomycin D effects on the concentrations of cadmium thionein, and copper chelatin in rat liver

The time courses of induction in rat liver of copper chelatin by copper, cadmium thionein by cadmium, and zinc thionein by copper, cadmium, and zinc were monitored following single intraperitoneal injections of metal salts. Low dosages of inducing metal were used in order to avoid toxic effects, being 5 mg zinc, 0.5 mg copper, and 0.25 mg cadmium per kg body weight. Peak times of induction and half times of decay observed were: copper chelatin (9 h, 8.6 h), cadmium thionein (18 h, 6.80 days), and zinc thionein (zinc rats, 18 h, 10.1 h; copper rats, 9 h, 18.2 h; cadmium rats, 24 h, 4.53 days). Administration of actinomycin D (1 mg per kg body weight) at the peak times of induction of the various proteins had no effect on the concentrations of chelatin or cadmium thionein observed up to 24 hours later, but in the case of zinc thionein, induced by zinc, copper, or cadmium, elevated concentrations were observed up to 23 h after administration of the drug. Such behavior is reminiscent of superinduction previously seen with other proteins and enzymes. We postulate that the intracellular concentration of free zinc in liver is of fundamental importance in the induction of zinc thionein, and this can be disturbed by exogenous copper or cadmium resulting in the induction of synthesis of zinc thionein.

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