a-巯基-β-芳基丙烯酸对大鼠肝脏低分子锌结合蛋白的诱导作用

Eugene Giroux, Beverly DaGue , N.J. Prakash
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引用次数: 4

摘要

腹腔注射α-巯基-β-(2-呋喃基)丙烯酸(MFA)诱导锌掺入鼠细胞质中低分子量的metailo蛋白部分。与MFA相比,α-巯基-β-(2-噻吩基)丙烯酸诱导的这部分锌的掺入较少。50 μmol/kg和200 μmol/kg MFA处理22 h后,大鼠血清总锌和放射性同位素锌浓度均明显高于单纯处理65ZnSO4的大鼠。此外,在mfat处理的大鼠肝脏中,通过凝胶层析解析的所有细胞质含锌蛋白中的放射性同位素锌浓度显著增加,但总锌仅在金属硫蛋白部分中升高,而在更高分子量的蛋白质中没有升高。这似乎是通过不涉及食物限制或金属盐管理的程序诱导金属硫蛋白样蛋白的第一个例子。
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Induction by a-Mercapto-β-arylacrylic Acids of Low-molecular-weight Zinc-binding Protein in Rat Liver

Intraperitoneal injections of α-mercapto-β-(2-furyl)acrylic acid (MFA) induced incorporation of zinc into a low-molecular-weight metailoprotein fraction in ratqiver cytosol. Cytosol of control rats contained little or none of this material, α-Mercapto-β-(2-thienyl)acrylic acid induced less incorporation of zinc in this fraction than did MFA. Twenty-two hours after administration of 65ZnSO4 (50 μmol/kg) and MFA (200 μmol/kg), serum concentrations of both total and radioisotopic zinc were markedly elevated, compared to serum concentrations in rats administered only 65ZNSO4. Further, in liversof MFAtreated rats, radioisotopic zinc concentration was significantly greater in all cytosol zinc-containing proteins resolved by gel chromatogzaphy, but total zinc was elevated only in the metallothionein fraction and not in proteins of higher molecular weight. This appears to be the first example of induction of a metallothionein-like protein by a procedure not involving food restriction or administration of metal salts.

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