产菌素乳酸菌的分离与鉴定

Nur Hidayah, Iman Rusmana, N. R. Mubarik
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引用次数: 0

摘要

本研究的目的是分离乳酸菌,并研究其抑制金黄色葡萄球菌和大肠杆菌生长的抑菌活性。对66株分离细菌进行筛选,发现2株乳酸菌可能产生细菌素。分离得到PB3.6和PG1.9,鉴定为植物乳杆菌和短乳杆菌。分离株PB3.6和PG1.9能产生细菌素,并能抑制金黄色葡萄球菌和大肠杆菌的生长。细菌素PB3.6和PG1.9分离物具有抑制食品腐败微生物生长的作用,有望作为食品中的替代防腐剂,从而减少化学添加剂材料的使用
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Isolation and Identification of potentially bacteriocin-producing lactic acid bacteria from Sawi Asin
The objective of this study are to isolate lactic acid bacteria from sawi asin and its antimicrobial activity in inhibiting the growth of Staphylococcus aureus and Escherichia coli. Screening of 66 bacterial isolates found 2 isolates of lactic acid bacteria that potentially produced bacteriocins. They were isolate PB3.6 and PG1.9, identified as Lactobacillus plantarum and Lactobacillus brevis, respectively. Isolates PB3.6 and PG1.9 could produced bacteriocins and able to inhibit the growth of S. aureus and E. coli. Bacteriocins of PB3.6 and PG1.9 isolates expected to be used as an alternative preservative agent in food products due to its capability of inhibiting the growth of food spoilage microbes, thus the usage of chemical additive material can be reduced
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