Pub Date : 2023-03-25DOI: 10.22487/25411969.2022.v11.i02.16217
A. Achyar, I. Putri, D. Putri
The existence of refillable drinking water depots helps the community to get affordable and practical drinking water. However, poor quality drinking water will, however, have an effect on health. One of the quality parameters of drinking water that is suitable for consumption is not contaminated by the bacteria Escherichia coli, Salmonella sp. and Escherichia sp. Measurement of the quality of drinking water, in addition to microbiological tests, can be carried out molecularly using PCR (Polymerase Chain Reaction) method. Therefore, the aim of this study was to examine the sensitivity and specificity of PCR for detection of drinking water pathogens. DNA was extracted from cultures of E. coli, Salmonella sp., Escherichia sp. and some non-coliform bacteria. PCR was performed separately using primer pairs of E. coli-AA-Forward and E.coli-AA-Reverse, Salmonella-OY-Forward and Salmonella-OY-Reverse, E. coli-DB-Forward and E. coli-DB-Reverse. The results of the PCR sensitivity showed that the minimum amount of DNA that can be detected by this method were 0.0025 ng/µL, 0,0005 ng/µL, 0,04 ng/µL for E. coli, Salmonella sp., Escherichia sp., respectively. The results of the PCR specificity of each primer pairs indicated that these methods were able to detect each coliform bacterium specifically according to PCR product size of ± 417 bp, ± 559 bp and ± 815 bp for E. coli, Salmonella sp., Escherichia sp., respectively.
可续水饮水站的存在帮助社区获得负担得起和实用的饮用水。然而，劣质饮用水会对健康产生影响。适于饮用的饮用水质量参数之一是不受大肠杆菌、沙门氏菌、埃希氏菌等细菌污染。饮用水质量的测定，除微生物学检测外，还可采用分子PCR (Polymerase Chain Reaction，聚合酶链反应)法进行。因此，本研究的目的是检验PCR检测饮用水病原体的敏感性和特异性。从大肠杆菌、沙门氏菌、埃希氏菌和一些非大肠菌群细菌培养物中提取DNA。分别用大肠杆菌- aa - forward和大肠杆菌- aa - reverse、沙门氏菌- y - forward和沙门氏菌- y - reverse、大肠杆菌- db - forward和大肠杆菌- db - reverse引物对进行PCR。结果表明，该方法对大肠杆菌、沙门氏菌、埃希氏菌的最低DNA检出量分别为0.0025 ng/µL、0,0005 ng/µL、0,04 ng/µL。各引物对的PCR特异性结果表明，该方法对大肠杆菌、沙门氏菌和埃希氏菌的PCR产物大小分别为±417 bp、±559 bp和±815 bp，均能特异性检测出大肠杆菌。
{"title":"Sensitivity and Specificity of Molecular-Based Pathogen Detection Method in Refillable Drinking Water Samples","authors":"A. Achyar, I. Putri, D. Putri","doi":"10.22487/25411969.2022.v11.i02.16217","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16217","url":null,"abstract":"The existence of refillable drinking water depots helps the community to get affordable and practical drinking water. However, poor quality drinking water will, however, have an effect on health. One of the quality parameters of drinking water that is suitable for consumption is not contaminated by the bacteria Escherichia coli, Salmonella sp. and Escherichia sp. Measurement of the quality of drinking water, in addition to microbiological tests, can be carried out molecularly using PCR (Polymerase Chain Reaction) method. Therefore, the aim of this study was to examine the sensitivity and specificity of PCR for detection of drinking water pathogens. DNA was extracted from cultures of E. coli, Salmonella sp., Escherichia sp. and some non-coliform bacteria. PCR was performed separately using primer pairs of E. coli-AA-Forward and E.coli-AA-Reverse, Salmonella-OY-Forward and Salmonella-OY-Reverse, E. coli-DB-Forward and E. coli-DB-Reverse. The results of the PCR sensitivity showed that the minimum amount of DNA that can be detected by this method were 0.0025 ng/µL, 0,0005 ng/µL, 0,04 ng/µL for E. coli, Salmonella sp., Escherichia sp., respectively. The results of the PCR specificity of each primer pairs indicated that these methods were able to detect each coliform bacterium specifically according to PCR product size of ± 417 bp, ± 559 bp and ± 815 bp for E. coli, Salmonella sp., Escherichia sp., respectively.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132372492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-25DOI: 10.22487/25411969.2022.v11.i02.16166
The Moringa oleifera can be used as a medium for the prevention and treatment of infectious diseases such as diarrhea. Diarrhea can be caused by Escherichia coli, which house flies can transmit. Moringa oleifera leaves have antimicrobial substances such as flavonoids, triterpenoids, steroids, saponins and tannins. This aim of the study was to determine the minimum inhibitory concentration of M. oleifera infusion on the growth of E. coli. This research is a laboratory experiment with Well Diffusion Agar method and dilution technique. The materials used in the study were M. oleifera leaf infusion with concentrations of 50%, 40%, 30%, 20%, and 10%, while 0% was a negative control (Aquadest). E. coli isolates were obtained from M. domestica. The results showed that the inhibition zone formed at concentrations of 50%, 40%, 30%, 20%, 10%, and 0% was 15.55 mm, 13.80 mm, 13.10 mm, 10.10 mm, and 0 mm, respectively. The minimum inhibitory concentration of leaf infusion against E. coli isolates was 25%, and the minimum bactericidal concentration could not be determined because all concentrations tested showed bacterial growth. Instead, the lowest inhibitory concentration was 25%. In concentrations of 50%, 40%, and 30%, M. oleifera leaf infusion has potent antibacterial action; at concentrations of 20%, it has moderate antimicrobial activity; and at concentrations of 10%, it is unable to inhibit the growth of E. coli. Further research is needed to determine the value of the minimum bactericidal concentration at concentrations above 25%, and it is necessary to test it on other digestive tract pathogenic bacteria.
{"title":"Antimicrobial activity of moringa leaf infusion on Escherichia coli isolate from Musca domestica L","authors":"","doi":"10.22487/25411969.2022.v11.i02.16166","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16166","url":null,"abstract":"The Moringa oleifera can be used as a medium for the prevention and treatment of infectious diseases such as diarrhea. Diarrhea can be caused by Escherichia coli, which house flies can transmit. Moringa oleifera leaves have antimicrobial substances such as flavonoids, triterpenoids, steroids, saponins and tannins. This aim of the study was to determine the minimum inhibitory concentration of M. oleifera infusion on the growth of E. coli. This research is a laboratory experiment with Well Diffusion Agar method and dilution technique. The materials used in the study were M. oleifera leaf infusion with concentrations of 50%, 40%, 30%, 20%, and 10%, while 0% was a negative control (Aquadest). E. coli isolates were obtained from M. domestica. The results showed that the inhibition zone formed at concentrations of 50%, 40%, 30%, 20%, 10%, and 0% was 15.55 mm, 13.80 mm, 13.10 mm, 10.10 mm, and 0 mm, respectively. The minimum inhibitory concentration of leaf infusion against E. coli isolates was 25%, and the minimum bactericidal concentration could not be determined because all concentrations tested showed bacterial growth. Instead, the lowest inhibitory concentration was 25%. In concentrations of 50%, 40%, and 30%, M. oleifera leaf infusion has potent antibacterial action; at concentrations of 20%, it has moderate antimicrobial activity; and at concentrations of 10%, it is unable to inhibit the growth of E. coli. Further research is needed to determine the value of the minimum bactericidal concentration at concentrations above 25%, and it is necessary to test it on other digestive tract pathogenic bacteria.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129127537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-25DOI: 10.22487/25411969.2022.v11.i02.16171
Wenny Pintalitna Tarigan, C. U. Tarigan, Cherline Halim, J. Valerie, Vionita Itanza, Russel Oeintz
Mucus from snakehead fish (Chana striata) and catfish (Clarias batrachus) has the potential to be employed in organic cosmetics and wound healing. The aim of this study is to create antimicrobials from Clarias batrachus and Chana striata mucus that may inhibit the growth of coliform bacteria. It also seeks to establish the minimum inhibitory concentration of Clarias batrachus and Chana striata mucus extracts against the growth of Escherichia coli when cocoa extract is added as an internal ingredient for making face masks and organic lipstick. The content of anthocyanin in cocoa beans used as a natural colouring agent that can replace synthetic dyes. The pooled fish mucus was extracted with succeeding centrifugation and filtration. The acidic mucus extracts were tested for antimicrobial-inhibitory effects and minimum inhibitory concentration (MIC) by agar-overlay diffusion and plate dilution method, respectively. The results showed that all fish mucus extracts exhibited antimicrobial effects against tested pathogens with catfish exhibiting the highest inhibitory effects against the bacteria sample as compared to the broad-spectrum antibiotic control. Interestingly, their mucus revealed inhibitory effects against bacteria at the lowest concentration (1:4 dilution). The present findings revealed the potential antimicrobial use of freshwater fish mucus against medically-important pathogens
{"title":"Antimicrobial Potential of Catfish (Clarias batrachus) and Snakehead Fish (Chana striata) Mucus on Bacterial Coliform Growth and its Application as the Organic Face Mask and Lipstick","authors":"Wenny Pintalitna Tarigan, C. U. Tarigan, Cherline Halim, J. Valerie, Vionita Itanza, Russel Oeintz","doi":"10.22487/25411969.2022.v11.i02.16171","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16171","url":null,"abstract":"Mucus from snakehead fish (Chana striata) and catfish (Clarias batrachus) has the potential to be employed in organic cosmetics and wound healing. The aim of this study is to create antimicrobials from Clarias batrachus and Chana striata mucus that may inhibit the growth of coliform bacteria. It also seeks to establish the minimum inhibitory concentration of Clarias batrachus and Chana striata mucus extracts against the growth of Escherichia coli when cocoa extract is added as an internal ingredient for making face masks and organic lipstick. The content of anthocyanin in cocoa beans used as a natural colouring agent that can replace synthetic dyes. The pooled fish mucus was extracted with succeeding centrifugation and filtration. The acidic mucus extracts were tested for antimicrobial-inhibitory effects and minimum inhibitory concentration (MIC) by agar-overlay diffusion and plate dilution method, respectively. The results showed that all fish mucus extracts exhibited antimicrobial effects against tested pathogens with catfish exhibiting the highest inhibitory effects against the bacteria sample as compared to the broad-spectrum antibiotic control. Interestingly, their mucus revealed inhibitory effects against bacteria at the lowest concentration (1:4 dilution). The present findings revealed the potential antimicrobial use of freshwater fish mucus against medically-important pathogens","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130043105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-08DOI: 10.22487/25411969.2022.v11.i02.16216
Y. Atifah, Afifatul Achyar
The use of heavy metal biomarkers is needed to detect and integrate heavy metals at the molecular level. Metallothionein gene is a gene that is expressed in fish organs that have been heavily polluted. The aim of the study was to find specific primers for methallothionein of tor fish. Detection of the metallothionein gene expression in PCR instruments requires a primer in the form of a short chain DNA sequence as a specific target DNA identifier. The primer design was performed in silico using the NCBI site and multiple-aligned using Geneious Prime bioinformatic software. Primers were designed according to the conserved region of these genes. The primers specificity was checked using Primer BLAST tools in NCBI. The results showed that the forward primer 1 (5'- GAT TGC GCC AAG ACT GGA ACT –3') and reverse primer 1 (5' – ATC ACG TTG ACC TCC TCA CTG -3') qualified as good primers with an amplicon size 186 bp.
{"title":"Design of Specific Primer for Methallothionein Gene of Tor Fish (Tor tambra)","authors":"Y. Atifah, Afifatul Achyar","doi":"10.22487/25411969.2022.v11.i02.16216","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16216","url":null,"abstract":"The use of heavy metal biomarkers is needed to detect and integrate heavy metals at the molecular level. Metallothionein gene is a gene that is expressed in fish organs that have been heavily polluted. The aim of the study was to find specific primers for methallothionein of tor fish. Detection of the metallothionein gene expression in PCR instruments requires a primer in the form of a short chain DNA sequence as a specific target DNA identifier. The primer design was performed in silico using the NCBI site and multiple-aligned using Geneious Prime bioinformatic software. Primers were designed according to the conserved region of these genes. The primers specificity was checked using Primer BLAST tools in NCBI. The results showed that the forward primer 1 (5'- GAT TGC GCC AAG ACT GGA ACT –3') and reverse primer 1 (5' – ATC ACG TTG ACC TCC TCA CTG -3') qualified as good primers with an amplicon size 186 bp.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115698959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tofu is a processed soybean food ingredient that is widely consumed by the public. Besides having a high nutritional content, tofu is also relatively cheap and easy to obtain. Tofu has a short shelf life and is easily damaged, so producers often add the preservative formaldehyde, which has been banned for use. Formalin or formaldehyde is not included in the list of food additives because they are toxic and harmful to health. This study was aimed to determine the reduction of formalin levels in white tofu by soaking green chilies. The study was carried out experimentally with the spectrophotometric method using the Schiff reagent. Absorbance measurements were carried out at a maximum wavelength of 580 nm. Parameters observed were the addition of 0% green chili solution concentration 0%; 2.5%; 5.0%; 7.5%; 10%; and 12.5% for 10 minutes of continuous immersion; 20 minutes; and 30 minutes. Based on the results of the study, it was found that each addition of green chili solution concentration reduced the formalin level along with the soaking time. The addition of 12.5% green chili solution for 30 minutes resulted in the greatest decrease in formalin levels, namely 69.82%. Based on these results it was concluded that the addition of green chili solution could reduce formalin in tofu.
{"title":"The Green chili to reduce formaldehyde in tofu by the spectrophotometric method using Schiff reagent","authors":"Edy Agustian Yazid, Roihatul Zahroh, Agita Anung Hadiyati","doi":"10.22487/25411969.2022.v11.i02.16144","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16144","url":null,"abstract":"Tofu is a processed soybean food ingredient that is widely consumed by the public. Besides having a high nutritional content, tofu is also relatively cheap and easy to obtain. Tofu has a short shelf life and is easily damaged, so producers often add the preservative formaldehyde, which has been banned for use. Formalin or formaldehyde is not included in the list of food additives because they are toxic and harmful to health. This study was aimed to determine the reduction of formalin levels in white tofu by soaking green chilies. The study was carried out experimentally with the spectrophotometric method using the Schiff reagent. Absorbance measurements were carried out at a maximum wavelength of 580 nm. Parameters observed were the addition of 0% green chili solution concentration 0%; 2.5%; 5.0%; 7.5%; 10%; and 12.5% for 10 minutes of continuous immersion; 20 minutes; and 30 minutes. Based on the results of the study, it was found that each addition of green chili solution concentration reduced the formalin level along with the soaking time. The addition of 12.5% green chili solution for 30 minutes resulted in the greatest decrease in formalin levels, namely 69.82%. Based on these results it was concluded that the addition of green chili solution could reduce formalin in tofu.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"591 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115104940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.22487/25411969.2022.v11.i02.16180
Devi Wulansari, Aripudin, Susi Ratnaningtyas, Sri Suminar Gopurullah
Seaweed has been widely used as a traditional medicinal ingredient by the community for generations. One species of seaweed, Eucheuma cottonii, is known that contains many antioxidant compounds. The antioxidant in seaweed is mainly in the form of polyphenolic antioxidative compounds, especially flavonoids. Flavonoid and tannin are two compounds that perform as antioxidant activity and have been considered as potential sunscreen ingredients. The test methods used in this study include SPF value determination test, sensitivity test, homogeneity test, pH test, and viscosity test. Sensitivity test was conducted by 20 semi-trained panelists. The data was statistically analyzed using one-way ANOVA SPSS version 21.0.
{"title":"Formulation and Physical Evaluation of Sunscreen Cream with Methanol Extract of Euchema cottonii","authors":"Devi Wulansari, Aripudin, Susi Ratnaningtyas, Sri Suminar Gopurullah","doi":"10.22487/25411969.2022.v11.i02.16180","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i02.16180","url":null,"abstract":"Seaweed has been widely used as a traditional medicinal ingredient by the community for generations. One species of seaweed, Eucheuma cottonii, is known that contains many antioxidant compounds. The antioxidant in seaweed is mainly in the form of polyphenolic antioxidative compounds, especially flavonoids. Flavonoid and tannin are two compounds that perform as antioxidant activity and have been considered as potential sunscreen ingredients. \u0000The test methods used in this study include SPF value determination test, sensitivity test, homogeneity test, pH test, and viscosity test. Sensitivity test was conducted by 20 semi-trained panelists. The data was statistically analyzed using one-way ANOVA SPSS version 21.0.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126301876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.22487/25411969.2022.v11.i01.15860
This study was aimed to examine the physical and chemical conditions of the seaweed growing areas in Morowali Regency. It was carried out in January–August 2019 in 13 sub-districts of Morowali Regency, however the sample was taken from 3 sub-districts, South Bungku District, Witaponda District, and Petasia District, because not all districts have seaweed cultivation locality. Data sampling was done in three observation stations, consisting of several research sub-stations. The result showed that only two of the fourteen criteria—carbon dioxide and nitrate—were found to be unfavorable for seaweed growth at three observation stations.
{"title":"Physical and chemical conditions of waters for seaweed cultivation in Morowali, Central Sulawesi, Indonesia","authors":"","doi":"10.22487/25411969.2022.v11.i01.15860","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i01.15860","url":null,"abstract":"This study was aimed to examine the physical and chemical conditions of the seaweed growing areas in Morowali Regency. It was carried out in January–August 2019 in 13 sub-districts of Morowali Regency, however the sample was taken from 3 sub-districts, South Bungku District, Witaponda District, and Petasia District, because not all districts have seaweed cultivation locality. Data sampling was done in three observation stations, consisting of several research sub-stations. The result showed that only two of the fourteen criteria—carbon dioxide and nitrate—were found to be unfavorable for seaweed growth at three observation stations.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116761681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.22487/25411969.2022.v11.i01.15696
Subaryanti Subaryanti, T. Triadiati, Y. C. Sulistyaningsih, D. I. Pradono
Kencur (Kaempferia galanga L.) is one of the potential medicinal plants with a high enough market demand so that the cultivation of kencur is still quite promising. The purpose of this study was to analyze the effect of altitude on the total phenol content of several accessions of kencur. The research was carried out at the Tropical Biopharmaceutical Laboratory, Bogor Agricultural University, from June to November 2017. The treatment tested was kencur accession rhizome taken from two altitudes, 214 m asl (lowlands) and 780 m asl (highlands). 10 g of kencur accession rhizome powder was added with methanol in a ratio of 1:10, then macerated while shaking using a magnetic stirrer (25 °C; 180 min). The extract was filtered and the filtrate was dried using a rotary vacuum evaporator (50 rpm; 60 °C) until a dry crude extract was obtained. Total phenol was tested by the Folin-Ciocalteu method at a wavelength of 730 nm. The results showed that altitude significantly affected the total phenol content of the kencur accession rhizome extract. The highest total phenol content value was obtained from Galesia 1 (GAL 1) in the lowlands of 17.92 mg/g (equivalent to gallic acid/GAE) and in the highlands obtained from PBG accession (Purbalingga) of 24.85 GAE mg/g compared to other accessions.
{"title":"Total phenol content of accessions of Kencur (Kaempferia galanga L.) at different altitudes","authors":"Subaryanti Subaryanti, T. Triadiati, Y. C. Sulistyaningsih, D. I. Pradono","doi":"10.22487/25411969.2022.v11.i01.15696","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i01.15696","url":null,"abstract":"Kencur (Kaempferia galanga L.) is one of the potential medicinal plants with a high enough market demand so that the cultivation of kencur is still quite promising. The purpose of this study was to analyze the effect of altitude on the total phenol content of several accessions of kencur. The research was carried out at the Tropical Biopharmaceutical Laboratory, Bogor Agricultural University, from June to November 2017. The treatment tested was kencur accession rhizome taken from two altitudes, 214 m asl (lowlands) and 780 m asl (highlands). 10 g of kencur accession rhizome powder was added with methanol in a ratio of 1:10, then macerated while shaking using a magnetic stirrer (25 °C; 180 min). The extract was filtered and the filtrate was dried using a rotary vacuum evaporator (50 rpm; 60 °C) until a dry crude extract was obtained. Total phenol was tested by the Folin-Ciocalteu method at a wavelength of 730 nm. The results showed that altitude significantly affected the total phenol content of the kencur accession rhizome extract. The highest total phenol content value was obtained from Galesia 1 (GAL 1) in the lowlands of 17.92 mg/g (equivalent to gallic acid/GAE) and in the highlands obtained from PBG accession (Purbalingga) of 24.85 GAE mg/g compared to other accessions.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128580118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.22487/25411969.2022.v11.i01.15699
Nova Rahma Widyaningrum, Sri Saptuti Wahyuningsih, Cipto Priyono
Muntingia calabura L. leaves extract are reported rich in flavonoidal compounds which shows a potential activity such as antidiabetic, antipyretic, analgesic and anti-inflammatory. Several subgroups of flavonoid compounds include flavones, flavanones, flavans and biflavans. This study aimed to analyze the anti-inflammatory activity of ethanolic, ethylacetate, and chloroform extracts of M. calabura L. leaves. The extraction process was carried out by maceration, where the yield of the three solvents were 19.14%; 8.41% and 6.59% w/w, respectively. The anti-inflammatory activity was carried out by observing a decrease in edema due to 1% carrageenan induction after 4 hours. The statistical results of anti-inflammatory activity showed that the ethanol extract had a significant difference with the positive control of p value < 0.05 in which a dose of 240 mg showed a decrease in edema volume (IEV) that was closed to the positive control (acetosal = 52.12%) which was 62.51% compared to the dose 60 mg and 120 mg. Furthermore, the % IEV of ethyl acetate extract of M. calabura L. leaves at a dose of 60, 120, and 240 mg were 36.30%, 26.83%, 24.24%, respectively. These results demonstrated that an average level of anti-inflammatory activity was 22.89%, and was closed to the dose of 500 mg/kg of acetosal-induced mice. Finally, the chloroform extract with the dose of 60 mg, 120 mg, 240 mg had a significant value of p < 0.05 with a positive control (acetosal). It could be concluded that the ethanol, ethylacetate and chloroform extracts of M. calabura L. leaves may have anti-inflammatory potential with acetosal as a comparison.
{"title":"Antiinflammation activity of Muntingia calabura L. leaves ethanol, ethylacetate and chloroform extracts","authors":"Nova Rahma Widyaningrum, Sri Saptuti Wahyuningsih, Cipto Priyono","doi":"10.22487/25411969.2022.v11.i01.15699","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i01.15699","url":null,"abstract":"Muntingia calabura L. leaves extract are reported rich in flavonoidal compounds which shows a potential activity such as antidiabetic, antipyretic, analgesic and anti-inflammatory. Several subgroups of flavonoid compounds include flavones, flavanones, flavans and biflavans. This study aimed to analyze the anti-inflammatory activity of ethanolic, ethylacetate, and chloroform extracts of M. calabura L. leaves. The extraction process was carried out by maceration, where the yield of the three solvents were 19.14%; 8.41% and 6.59% w/w, respectively. The anti-inflammatory activity was carried out by observing a decrease in edema due to 1% carrageenan induction after 4 hours. The statistical results of anti-inflammatory activity showed that the ethanol extract had a significant difference with the positive control of p value < 0.05 in which a dose of 240 mg showed a decrease in edema volume (IEV) that was closed to the positive control (acetosal = 52.12%) which was 62.51% compared to the dose 60 mg and 120 mg. Furthermore, the % IEV of ethyl acetate extract of M. calabura L. leaves at a dose of 60, 120, and 240 mg were 36.30%, 26.83%, 24.24%, respectively. These results demonstrated that an average level of anti-inflammatory activity was 22.89%, and was closed to the dose of 500 mg/kg of acetosal-induced mice. Finally, the chloroform extract with the dose of 60 mg, 120 mg, 240 mg had a significant value of p < 0.05 with a positive control (acetosal). It could be concluded that the ethanol, ethylacetate and chloroform extracts of M. calabura L. leaves may have anti-inflammatory potential with acetosal as a comparison.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133693485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.22487/25411969.2022.v11.i01.15853
G. M. Nurdin, Nur Hidayah, Naura Nazifa R. Hidayat, Erpi Nurdin
This study aimed to determine the hand antiseptic products effectivity against the growth of Staphylococcus aureus bacteria. The sample used were hand antiseptic products purchased from several minimarkets, markets, and obtained from several institutions in West Sulawesi by using purposive sampling technique. Samples were evaluated using the well diffusion method with three replications. Since the study data did not meet the ANOVA test requirements, the Kruskal-Wallis analysis test was used to analyse the data, then was continued with the Mann Whitney test. The results showed the sig. value of 0.00 < 0.05 which means, there was a significant difference between hand antiseptics in inhibiting the growth of Staphylococcus aureus bacteria. The results showed that only 2 out of 10 hand antiseptic products with codes N8 and N10 were effective in inhibiting the growth of Staphylococcus aureus bacteria. This can be seen from the formation of a clear zone around the hand antiseptic.
{"title":"The effectivity of hand antiseptic against Staphylococcus aureus","authors":"G. M. Nurdin, Nur Hidayah, Naura Nazifa R. Hidayat, Erpi Nurdin","doi":"10.22487/25411969.2022.v11.i01.15853","DOIUrl":"https://doi.org/10.22487/25411969.2022.v11.i01.15853","url":null,"abstract":"This study aimed to determine the hand antiseptic products effectivity against the growth of Staphylococcus aureus bacteria. The sample used were hand antiseptic products purchased from several minimarkets, markets, and obtained from several institutions in West Sulawesi by using purposive sampling technique. Samples were evaluated using the well diffusion method with three replications. Since the study data did not meet the ANOVA test requirements, the Kruskal-Wallis analysis test was used to analyse the data, then was continued with the Mann Whitney test. The results showed the sig. value of 0.00 < 0.05 which means, there was a significant difference between hand antiseptics in inhibiting the growth of Staphylococcus aureus bacteria. The results showed that only 2 out of 10 hand antiseptic products with codes N8 and N10 were effective in inhibiting the growth of Staphylococcus aureus bacteria. This can be seen from the formation of a clear zone around the hand antiseptic.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130809277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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