Purification and Antibacterial Activity of Marine Actinomycetes against Human and Fish Pathogens

Many actinomycetes strains were isolated from soil samples of Thiruvananthapuram District, Kerala. The isolated strains were identified based on their morphological, cultural and biochemical characteristics. Totally thirty actinomycetes species were isolated and used for antibacterial activity. Out of thirty actinomycetes species, Streptomyces spp., showed the best level of antibacterial activity against human and fish pathogens. The bioactive compound protein was partially purified and tested for antibacterial activity. The partially purified bioactive compound was subjected for SDS-PAGE for protein profiling and band was obtained. The partially purified protein was allowed for High Performance Liquid Chromatography analysis and was tested for antibacterial activity against human and fish pathogens. The purified protein again subjected to SDS-PAGE analysis.