基于二硫化钼/石墨烯的电化学生物传感器高灵敏度检测人甲状旁腺激素

H. Kim, Kook-Nyung Lee, Min-Ho Lee, Hyeong-U. Kim, Chisung Ahn, Taesung Kim
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引用次数: 1

摘要

在金(Au)电极上静电形成了由mos2 -石墨烯纳米片组成的自组装纳米复合材料(MoS2-GNS),并报道了其生化扩增反应。采用扫描电子显微镜(SEM)、高分辨率透射电子显微镜(HR-TEM)、x射线衍射仪(XRD)和傅里叶变换红外光谱仪(FT-IR)对MoS2-GNS的性质进行了表征。循环伏安法在甲状旁腺激素(PTH)存在下进行,通过底物和酶偶联二抗测量MoS2-GNS-Ab与不同浓度甲状旁腺激素相互作用产生的阳极电流信号,得到其线性关系。
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Electrochemical biosensor based on MoS2/Graphene for highly sensitive detection of human parathyroid hormone
A self-assembled novel nanocomposite composed of MoS2-Graphene nanosheets (MoS2-GNS) composites were electrostatically formed on top of gold (Au) electrode and their biochemical amplification responses were reported. The properties of the MoS2-GNS were characterized by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), and Fourier transform infrared spectrometer (FT-IR). Cyclic voltammetry was performed in the presence of Parathyroid Hormone (PTH) and its linearity was obtained by measuring the anodic current signals coming from interactions between MoS2-GNS-Ab and different concentrations of PTH with the aid of substrate and enzyme conjugated secondary antibodies.
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