{"title":"芒廷叶甲醇提取物对胶原蛋白生成的促进作用","authors":"A. Rahmawati, O. P. Astirin, A. Pangastuti","doi":"10.1063/1.5139830","DOIUrl":null,"url":null,"abstract":"Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.","PeriodicalId":246056,"journal":{"name":"THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION","volume":"77 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"The effect of Muntingia calabura L. leaves methanolic extract in increasing of collagen production\",\"authors\":\"A. Rahmawati, O. P. Astirin, A. Pangastuti\",\"doi\":\"10.1063/1.5139830\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.\",\"PeriodicalId\":246056,\"journal\":{\"name\":\"THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION\",\"volume\":\"77 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-12-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1063/1.5139830\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1063/1.5139830","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The effect of Muntingia calabura L. leaves methanolic extract in increasing of collagen production
Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.
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