富Oh自由基常压等离子体对反渗透水的消毒

A. Dória, Gislene S. Liberato, F. R. Figueira, C. A. Carvalho, J. B. S. Lima, R. Pessoa, S. Khouri
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引用次数: 1

摘要

反渗透水是血液透析治疗的主要成分之一,其化学和微生物特性对于避免对患者的额外风险至关重要。2009年至2011年期间在美国进行的一项研究显示,在透析的头180天内,患者因感染而死亡率约为9.6% 1。在大气压下操作的非热等离子体技术正在获得一种根除和控制细菌和真菌污染的潜在方法。本研究旨在评价不同大气等离子体射流对含有标准菌株ATCC (American Type Culture Collection)的大肠埃希菌(10798)、变形杆菌(35659)和肺炎克雷伯菌(31488)的反渗透水的消毒效果。方法采用反渗透水制备0.5倍Mac - Farland标度的细菌接种剂。将样本分为三组:(A)对照组;(B) 5L / min氩气和水蒸气等离子体处理;(C) 5L / min氩气和过氧化氢等离子体处理。处理一式三次,每次10分钟,在喷嘴和液体表面之间保持1cm的距离。采用滑动电弧放电反应器产生等离子体射流。对于等离子体的分析,我们使用了光学发射光谱(OES)技术,以表征其化学性质。两种等离子体组合物对不同微生物污染的反渗透水均有较好的消毒效果,CFU / mL可降低99.9%,OES光谱显示OH强度提高了2个数量级。
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Disinfection Of Reverse Osmosis Water By Atmospheric Plasma Rich In Oh Radical
Reverse osmosis water is one of the main components of hemodialysis treatment and its chemical and microbiological qualities are vital to avoid additional risks to the patient. A study conducted in the United States between 2009-2011 showed a mortality rate of patients in the first 180 days of dialysis, due to infections, of around 9.6% 1. The technology of non-thermal plasmas, operated under atmospheric pressure, is gaining a potential approach in the eradication and control of bacterial and fungal contamination 2. The aim of this work is to evaluate the efficiency of different atmospheric plasma jet on the disinfection of reverse osmosis water contaminated with standard strains ATCC (American Type Culture Collection) of Escherichia coli(10798), Proteus Mirabilis(35659) and Klebsiella pneumoniae(31488). In the methodology, were prepared bacterial inoculums with reverse osmosis water on the 0.5 of Mac Farland scale. The samples were divided into three groups: (A) control; (B) treated with 5L / min plasma of Argon and water vapor; (C) treated with 5L / min plasma of Argon and hydrogen peroxide. Treatments were performed in triplicate for 10 minutes and at a distance of 1 cm between the nozzle and the liquid surface. A gliding arc discharge reactor was used to generate the plasma jet. For the analysis of the plasma, we used the optical emission spectroscopy (OES) technique, in order to characterize its chemistry. The two plasma compositions used were efficient in the disinfection of the reverse osmosis water contaminated with the different microorganisms, reaching 99.9% reduction of CFU / mL. OES spectra show an increase of 2 orders of magnitude in the OH intensity.
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