半闭式微芯片内的热凝胶驱动喷口/吸口装置

Masaru Takeuchi, M. Nakajima, T. Fukuda
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引用次数: 1

摘要

本文提出了一种利用热凝胶的体积变化作为致动器的热凝胶致动探针。热凝胶驱动探针可用于单细胞分析,特别是单细胞操作。从溶胶到凝胶的相变使体积增大,而从凝胶到溶胶的相变使体积减小。热凝胶驱动探针可以通过这些体积变化喷出和吸入溶液。这种热凝胶驱动探针可以作为一次性设备使用。热凝胶驱动探针还实现了易于使用,因为它只需要打开和关闭加热器的喷嘴和吸入溶液。对热凝胶的体积变化进行了评价。并对热凝胶驱动探针喷出溶液的体积进行了评价。最后,利用热凝胶驱动探针对目标单微珠的吸力和喷出进行了验证。认为热凝胶驱动探针可以实现靶细胞的操作,有助于单细胞分析。
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Thermal gel actuated device for spout/suction inside semi-closed microchip
In this paper, we propose a thermal gel actuated probe which uses the volume change of the thermal gel as an actuator. The thermal gel actuated probe can be used for single cell analysis, especially single cell manipulation. The phase change from sol to gel causes an increase in volume, while the change from gel to sol causes a decrease in volume. The thermal gel actuated probe can spout and suck solution by these volume changes. This thermal gel actuated probe can be used as a disposable device. The thermal gel actuated probe also realize easy to use because it is needed only to switch the heater turning on and off for spout and suction of solution. The volume change of the thermal gel was evaluated. It is also evaluated that the volume of spouted solution by the thermal gel actuated probe. Finally, the target single microbead suction and spout were demonstrated by the thermal gel actuated probe. It is considered that the thermal gel actuated probe can realize the target cell manipulation, and can contribute to the single cell analysis.
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