补充热杀菌细胞对玉米作物基质上蘑菇菌生长的影响

Shivabasu Khanagoudar, B. Mallesha
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引用次数: 0

摘要

以玉米秸秆、玉米芯鞘和玉米皮为原料,进行了平菇(Pleurotus eoeus)的栽培试验。初步研究了热灭菌分离物(Iso-S-1、Iso-S-2、Iso-S-3、Iso-Sh-4、Iso-R-5)对杏鲍菇体外生长的影响。热灭菌分离物(Iso-S-3、Iso-R-5)对真菌有促进生长的作用。因此,所选的两株细菌将用于进一步的研究。Iso-S-3和Iso-R-5在营养液上生长,然后在121℃灭菌15分钟。然后将100 ml无菌细胞悬浮液喷洒在平均重量2.5 ~ 3.0 kg的湿基巴氏杀菌玉米作物副产品和水稻秸秆上。与未处理的基质相比,添加了热杀细菌细胞(Iso-S-3)的玉米秸秆基质和水稻秸秆基质能促进平菇的最高生长。
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EFFECT OF HEAT KILLED BACTERIAL CELL SUPPLIMENTATION FOR GROWTH OF MUSHROOM FUNGUS ( PLEUROTUS EOEUS ) ON MAIZE CROP SUBSTRATE
The research was carried out to investigate the cultivation of oyster mushroom ( Pleurotus eoeus ) on maize crop byproducts (maize Stover, maize cob sheath and maize rind). The preliminary test was done to know the effect of heat killed bacterial isolates (Iso-S-1, Iso-S-2, Iso-S-3, Iso-Sh-4, Iso-R-5) on the growth of Pleurotus eoeus in in vitro . Among the heat killed bacterial isolates (Iso-S-3, Iso-R-5) showed the growth stimulatory character on mushroom fungus. So that the selected two bacterial isolates were used for the further study. The Iso-S-3 and Iso-R-5 was grown on nutrient broth then sterilized at 121 0 C for 15 minutes. Then the 100 ml sterilized cell suspended broth was sprayed on the average weight of 2.5-3.0 kg wet base pasteurized maize crop byproducts and paddy straw. This heat killed bacterial cell supplementation (Iso-S-3) enhances the highest growth of Pleurotus eoeus on maize stover substrate and paddy straw compared to the untreated substrates.
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