STUDY OF THE HEPATOPROTECTIVE EFFECT OF EXTRACTS FROM NATIVE AND DISPERSED BIRCH BARK IN CASE OF CARBON TETRACHLODIDE INTOXICATION

The hepatoprotective effect of aqueous alcoholic extracts obtained from the raw materials of native birch bark (NBBE) and previously dispersed to a particle size of 100–300 nm (DBBE) was studied in male rats. The animals were divided into 14 groups of 10 animals each. Groups 1, 2 — intact; 3, 4 — control (carbon tetrachloride); 5, 6 — previously, for 5 days, NBBE was injected once intragastrically at a dose of 50 mg/kg, on the 6th day, a 50 % oil solution of CC14 was injected at a dose of 0,2 ml/100 g abdominally; 7, 8 — DBBE at a dose of 50 mg/kg + CC14; 9, 10 — NBBE at a dose of 100 mg/kg + CC14; 11, 12 — DBBE at a dose of 100 mg/kg + CC14; 13, 14 — the comparison drug carsil was injected at a dose of 100 mg/kg + CC14. On the 4th and 7th day after the injection of CC14, the animals were removed from the experiment. The data obtained indicate the hepatoprotective effect of NBBE and DBBE in carbon tetrachloride intoxication, which was expressed in the necrosis volume density decrease due to their preliminary preventive injection. NBBE at a dose of 100 mg/kg has a more significant protective effect compared to Carsil. Prophylactic injection of DBBE at doses of 50 and 100 mg/kg not only prevented the development of necrosis in the liver, but unlike Carsil, it activated intracellular processes of reparative regeneration in the recovery period, which is confirmed by an increase in the number density of binuclear hepatocytes.