DETECTION OF SOME VIRULENCE FACTORS AND ANTIBIOTICS RESISTANCE OF KLEBSIELLA PNEUMONIAE

Background: Infections of Klebsiella pneumoniae can include; diarrhea, septicemia, pneumonia, urinary tract infection and infections of soft tissues. Many factors are donated to K. pneumoniae pathogenicity particularly production of enzymes and formation of biofilm. Objective: find the relationship between the resistance of K. pneumoniae bacteria to antibiotics of quinolones and their ability to produce enzymes of beta lactamase. Materials and Methods: The Study included isolation and identification of (51) isolate of K. pneumoniae and (94) isolates of other bacteria from different clinical sources in some Baghdad hospitals. Results: The isolation and diagnosis of (51) isolates of K. pneumoniae from infection of urinary tract were 49.1%, infection of wounds were 31.3% and infection of burns were19.6%. All bacterial isolates were identified by the biochemical, cultural and microbial characteristics and confirmed by Api E20 System. I showed of β-lactamase test of Klebsiella pneumoniae revealed that (35) 68.6% isolates were positive. While 16 (31.4%) isolates were able to produce urease. Four groups of quinolones were tested by done the sensitivity test of isolates and results revealed the following percentage of resistant to Norfloxacin, Ofloxacin and ciprofloxacin consequently were (50.1%), (44.5%), (39.4%). whereas, the lower percent of resistant to Levofloxacin was (26.8%). In contrast, the βlactamase positive K. pneumoniae exhibited a high resistance in compare to isolates that negative for β-lactamase. The minimum inhibitory range concentrations of ciprofloxacin were arranged between (4-512 µg\ml). From isolates that resistant to Ciprofloxacin, the DNA plasmid was determined. Single plasmid bands were included in two isolates with same size and other isolates were confined free plasmid