猪舍分离的福氏志贺氏菌和粪肠球菌的抗菌光谱、生长/杀伤动力学、常规/分子分析和紫外光谱特征

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引用次数: 2

摘要

该研究的目的是获取猪舍中微生物的负荷和微生物,并比较猪舍分离物的传统方法和分子方法(MEGA 6a rDNA测序)。利用紫外分光光度计对菌株的抗菌谱、生长/杀伤动力学进行了评价。采用无菌换棒法在猪舍墙[w]和2km处、猪体[B]和地板[F]处取样。对样品进行连续稀释,用营养琼脂进行传代培养,并进行生化试验作为初步试验。初步检测鉴定出结核分枝杆菌、炭疽芽孢杆菌、蜡样芽孢杆菌、金黄色葡萄球菌、梭状芽孢杆菌,并采用Kirby-bauer药敏盘进行抗生素药敏试验(amtibigram)。所有分离株均对环丙沙星、左氧氟沙星、庆大霉素、利福平、链霉素、红霉素和阿莫索敏感。分离株对诺氟沙星、氯霉素和氨苄氧苄啶耐药。对三个分离株进行了分子测序以进行确证试验。检出福氏志贺氏菌和粪肠球菌。用紫外分光光度计测定猪舍分离株的生长速率和死亡率/杀灭时间。在波长480λ处观测到。芽孢杆菌的生长速率最高,为0.525λ,枯草芽孢杆菌的生长速率最低,为0.001λ。在第84 h,芽孢杆菌的死亡率最低,为0.307 λ,蜡样芽孢杆菌的死亡率最高,为0.227λ;在第24 h,用紫外分光光度法测定了分离菌的生长动态和杀灭时间以及添加环丙沙星抗生素的情况。在0 h时,枯草芽孢杆菌的生长速率最高,为0.251λ,蜡样芽孢杆菌的生长速率最低,为0.019λ。第84 h,枯草芽孢杆菌死亡率最低,为0.152λ,枯草芽孢杆菌死亡率最高,为0.097 λ。猪舍和动物的适当卫生可以帮助尽量减少猪舍内可能发现的微生物,这些微生物可能对食用猪的人和猪舍内的农民构成主要健康危害。当食用受感染动物的猪肉时,它也可以作为食源性病原体,构成潜在的健康危害。
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Antimicrobial Spectrum, Growth/ killing kinetics, Conventional/Molecular assay and Ultraviolet Spectrophotometer Signatures of Characterizing Shigella Flexneri and Enterococcus Faecalis and Isolated from Swine House isolates
The objective of the study was to access microbial load and microorganism found in swine house depending on the sample site and to compare between the conventional and molecular methods (MEGA 6a rDNA sequencing) of characterization of swine house isolates. The antimicrobial spectrum, growth/ killing kinetics of the isolates using Ultraviolet spectrophotometer signatures were also evaluated. The sample were taken at the pig house from the wall [w] and at a distance of 2km and body[B] and floor[F] using sterile swap stick. The sample underwent serial dilution and a pure isolate was sub-cultured using nutrient agar and also biochemical test was conducted as a preliminary test. From the preliminary test, the following organism were identified, Mycobacterium tuberculosis, Bacillus spp Bacillus anthracis, Bacillus cereus, Staphylococcus aureus, Clostridium sp. In addition the samples were tested for antibiotics susceptibility test (Amtibiogram) using Kirby-bauer antibiotic susceptibility disc. All isolates were found to be susceptible to Ciprofloxacin, Levofloxacin, Gentamycin, Rifampicin, Streptomycin, Erythromycin and Amoxyl. Isolates were resistant to Norflaxacin, Chloramphenicol and Ampiclox. Molecular sequencing were performed on three isolates for a confirmatory test. It was observed that Shigella flexneri and Enterococcus faecalis. Growth rate and death rate / killing time of isolates using ultraviolet spectrophotometer from the swine house were measured. It was observed, At, wavelength 480λ. Bacillus spp has the highest growth rate of 0.525λ and Bacillus subtilis have the lowest growth rate of 0.001λ. At 84th hour, bacillus spp has the lowest death rate of 0.307 λ and Bacillus cereus have the highest death rate of 0.227λ, growth dynamic and killing time of bacteria isolates and addition of ciprofloxacin antibiotic at 24th hour using ultraviolet spectrophotometer. it was observed that at 0 hour, Bacillus subtilis has the highest growth rate of 0.251λ and Bacillus cereus have the lowest growth rate of 0.019λ. At the 84th hour, Bacillus kaustophilus has the lowest death rate of 0.152λ and Bacillus subtilis have the highest death rate of 0.097. Proper sanitation of pig house as well as the animals can help minimize the possible organisms found in the swine house which may serve as a major health hazards for people that consume pig and farmers in the pig house. It can also serve as food-borne pathogen posing potential health hazard when pork from infected animals are consumed.
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