{"title":"里氏木霉内切葡聚糖酶2的克隆及生物信息学分析","authors":"Yanling Yang, Lihua Liu, Miao Diao, Zhiwei Lin, Wunian Guo, Shihua Wang","doi":"10.1109/WCSE.2009.175","DOIUrl":null,"url":null,"abstract":"Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using some online or offline services.","PeriodicalId":331155,"journal":{"name":"2009 WRI World Congress on Software Engineering","volume":"332 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2009-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cloning and Bioinformatics Analysis of an Endoglucanase 2 from Trichoderma reesei\",\"authors\":\"Yanling Yang, Lihua Liu, Miao Diao, Zhiwei Lin, Wunian Guo, Shihua Wang\",\"doi\":\"10.1109/WCSE.2009.175\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using some online or offline services.\",\"PeriodicalId\":331155,\"journal\":{\"name\":\"2009 WRI World Congress on Software Engineering\",\"volume\":\"332 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2009 WRI World Congress on Software Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/WCSE.2009.175\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2009 WRI World Congress on Software Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/WCSE.2009.175","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cloning and Bioinformatics Analysis of an Endoglucanase 2 from Trichoderma reesei
Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using some online or offline services.
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