植物pks和fas途径的生物信息学分析及外源性诱导剂对离体培养番石榴中乙酰原素生产的影响

S. Sánchez-Ramos, Felipe Guadalupe Carrillo-Landell, Gustavo Cruz-Jiménez, Alan Alexander González-Ibarra
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引用次数: 0

摘要

的目标。从生物信息学的角度分析外源诱导剂对临时浸没生物反应器(BITs)培养的村田南(Annona muricata)产乙酰原素的影响。方法。采用生物信息学方法对产醋原的聚酮合成酶(PKS)和脂肪酸合成酶(FAS)植物的代谢途径进行了KEGG位点的分析。以番荔枝为外植体进行5次处理;1) BAP, 2) BAP + AAs, 3) BAP + AAs +腐胺,4)BAP + IAA, 5) BAP + IAA +柠檬酸盐。BAP和IAA是植物激素,AAs是3种生酮氨基酸的混合物,腐胺和柠檬酸盐是PKS和FAS的诱导剂。的贡献。在无明显污染的情况下,对5个处理的外植体培养进行了8周的BITs监测。处理1和处理2从第6周开始萌芽观察,但处理3超过8周没有萌芽观察,腐胺在外植体坏死过程中不仅仅是诱导剂和应激因子。处理4和处理5只监测到第三周,未收集发芽信息。用高效液相色谱- pda法观察了离体、离体处理和处理1、处理2、处理3之间醋原素谱的差异。
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Análisis bioinformático de las rutas pks y fas en plantas y uso de inductores exógenos sobre la producción de acetogeninas en guanábana cultivada in vitro
Goals. To analyze the effect on the production of acetogenins of Annona muricata cultivated in vitro by means of Temporary Immersion Bioreactors (BITs) promoted by the use of exogenous inducers proposed from a bioinformatic analysis. Methodology. A bioinformatic analysis was carried out at the KEGG site of the metabolic pathways of polyketide synthetase (PKS) and fatty acid synthetase (FAS) plants from which acetogenins are derived. Five treatments were performed in the BITs with soursop explants; 1) BAP, 2) BAP + AAs 3) BAP + AAs + Putrescine, 4) BAP + IAA and 5) BAP + IAA + Citrate. BAP and IAA are plant hormones, AAs is a mixture of three ketogenic amino acids, Putrescine and Citrate are inducers of PKS and FAS. Contribution. The cultivation of soursop explants was monitored using BITs of the 5 treatments for 8 weeks without apparent contamination. Sprout observation started from week 6 in treatment 1 and 2 but not in 3 over 8 weeks, with putrescine being more than an inducer a stress factor for the explant as they became necrotic. Treatments 4 and 5 were only monitored until the third week and no information on sprouting was collected. Differences in the profile of acetogenins by HPLC-PDA were observed between soursop leaves to ex vitro and in vitro and between treatments 1, 2 and 3.
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