科尼亚灌溉条件下不同基因型燕麦饲料产量的研究

M. A. Qureshi, W. Shafqat
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摘要

以芒果新芽的茎尖为外植体,在MS培养基上直接培养。在MS基础培养基中添加不同浓度的BAP、NAA和IAA等植物生长调节剂。试验采用完全随机设计(CRD),共设4个处理,每个处理重复3次。BAP浓度为1 mg/L时芽诱导率最高,达到55.56%。最大BAP浓度为3 mg/L时,死亡率最高,为83.30%。在添加1 mg/L BAP的MS培养基上,诱导芽的最短时间为12.633天。在添加1 mg/L BAP的MS培养基中,芽数最多,为2.50个。与高浓度NAA和IAA相比,低浓度NAA在再生芽中较早形成根。添加NAA (1 mg/L)的MS培养基诱导生根的时间较短,为21天。生长素组合(MS+ NAA 3 mg /L + IAA 1 mg /L)诱导生根效果最佳(38.87%)。关键词:芒果,热带水果,无性繁殖,植物组织培养,微繁,酚类分泌物,驯化
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INVESTIGATIONS ON FORAGE YIELD OF OAT GENOTYTPES UNDER IRRIGATED CONDITIONS OF KONYA
Shoot tips of newly emerging mango shoots were used as an explant and cultured on MS media for direct shooting. Different plant growth regulators i.e. BAP, NAA and IAA in varying concentrations were added to basal MS media. Experiment was laid out according to Completely Randomized Design (CRD) with four treatments and each treatment was replicated thrice. Maximum shoot induction (55.56%) was observed with 1 mg/L of BAP. Maximum mortality (83.30%) was observed when maximum concentration of BAP (3 mg/L) was used. Minimum days (12.633) to induce shoots were observed on MS media supplemented with 1 mg/L of BAP. Maximum number of shoots (2.50) were recorded in MS media supplemented with 1 mg/L of BAP. Low concentration of NAA initiated roots earlier in regenerated shoots as compare to high concentrations of NAA and IAA. MS media supplemented with NAA (1 mg/L) took less days (21 days) to induce roots. The combination of auxins (MS+ NAA 3 mg /L + IAA 1 mg /L) proved the best for root induction (38.87%). Keywords: Mangifera indica, tropical fruits, asexual propagation, plant tissue culture, micropropagation, phenolic exudation, acclimatization.
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