Peter M. Lydyard, Michael F. Cole, John Holton, William L. Irving, Nino Porakishvili, Pradhib Venkatesan, K. Ward
{"title":"伯纳特氏立克次氏体","authors":"Peter M. Lydyard, Michael F. Cole, John Holton, William L. Irving, Nino Porakishvili, Pradhib Venkatesan, K. Ward","doi":"10.4324/9780203856871-6","DOIUrl":null,"url":null,"abstract":"% (goats). Detection of Coxiella burnetii DNA was determined through use of a real time PCR assay validated for use in bovine, ovine, and caprine feces; threshold of detection is one DNA copy per PCR (sensitivity 95.8%, specificity 100%). All tested samples were negative for Coxiella burnetii DNA. We conclude that non‐dairy, non‐periparturient ruminants exhibited at Iowa fairs are unlikely to shed Coxiella burnetii in their feces and that this population should not be considered to be a significant exposure risk to other livestock or fair attendees.","PeriodicalId":370939,"journal":{"name":"Case Studies in Infectious Disease","volume":"65 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Coxiella burnetii\",\"authors\":\"Peter M. Lydyard, Michael F. Cole, John Holton, William L. Irving, Nino Porakishvili, Pradhib Venkatesan, K. Ward\",\"doi\":\"10.4324/9780203856871-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"% (goats). Detection of Coxiella burnetii DNA was determined through use of a real time PCR assay validated for use in bovine, ovine, and caprine feces; threshold of detection is one DNA copy per PCR (sensitivity 95.8%, specificity 100%). All tested samples were negative for Coxiella burnetii DNA. We conclude that non‐dairy, non‐periparturient ruminants exhibited at Iowa fairs are unlikely to shed Coxiella burnetii in their feces and that this population should not be considered to be a significant exposure risk to other livestock or fair attendees.\",\"PeriodicalId\":370939,\"journal\":{\"name\":\"Case Studies in Infectious Disease\",\"volume\":\"65 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Case Studies in Infectious Disease\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4324/9780203856871-6\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Case Studies in Infectious Disease","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4324/9780203856871-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
% (goats). Detection of Coxiella burnetii DNA was determined through use of a real time PCR assay validated for use in bovine, ovine, and caprine feces; threshold of detection is one DNA copy per PCR (sensitivity 95.8%, specificity 100%). All tested samples were negative for Coxiella burnetii DNA. We conclude that non‐dairy, non‐periparturient ruminants exhibited at Iowa fairs are unlikely to shed Coxiella burnetii in their feces and that this population should not be considered to be a significant exposure risk to other livestock or fair attendees.
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