象草生物质水解产率及木质纤维素乙醇的生产

Lara Neiva de Siqueira, E. Guarda, P. M. Guarda, R. F. D. Silva, R. Barbosa
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摘要

为了寻找易于获取、成本低、纤维素浓度高的替代生物质,用于生产第二代乙醇或木质纤维素,本研究以象草Pennisetum purpureum进行。本研究测定了象草料的化学成分,以评价其在生产木质纤维素乙醇中的应用。2015年8月在费拉帕尔马斯农业技术试验区收获象草茎。结果表明,象草含有约60%的纤维素,表明这种生物质可以用于获取还原糖。在酶解步骤之前,生物质经过化学预处理,使用商用纤维素酶Cellic CTEC2。水解后还原糖得率为54%,经水解后还原糖得率为53.9%。此外,乙醇的产率为87,8%。因此,可以肯定的是,象草生物质作为纤维素来源具有巨大的潜力,因此它可以作为一种廉价和大量可用的替代品用于生产木质纤维素乙醇。
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Yield of hydrolysis and production of lignocellulosic ethanol from elephant grass biomass
Due to search for alternative biomasses, that are easy to access, low cost and high in cellulose concentration, for the production of 2nd generation ethanol - or lignocellulosic - this study was carried out with Pennisetum purpureum, known as elephant grass. The study was conducted to determine the chemical composition of elephant grass forage to evaluate its use in the production of lignocellulosic ethanol. The elephant grass stems were reaped in the experimental area of Feira Agrotecnologica de Palmas (AGROTINS) on august 2015. The results have shown that the elephant grass presented approximately 60% cellulose, demonstrating the potential use of this biomass to obtain reducing sugars. The biomass underwent chemical pretreatment before the enzymatic hydrolysis step using commercial cellulase enzyme Cellic CTEC2. After hydrolysis, gave a 54% yield of reducing sugars, which has shown a yield of 53,9% in the hydrolysis. In addition, the yield for ethanol production was 87,8%. Due to this, it is possible to assure that the elephant grass biomass has a great potential as cellulose source, therefore it can be used as an alternative that is cheap and huge in availability to the production of lignocellulosic ethanol.
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