{"title":"一种用于血清细菌检测的高灵敏度微流控装置","authors":"M. Al-Adhami, E. Tan, G. Rao, Y. Rostov","doi":"10.1109/HIC.2017.8227601","DOIUrl":null,"url":null,"abstract":"Highly sensitive device to detect bacteria in blood serum is presented. The device comprises of a microfluidic component and an electronic reader. The microfluidic cassette acts as an enclosed vial. It is filled with the sample after mixing with an indicator dye. Then, it is inserted into a kinetics fluorometer. The rate of the fluorescence increase is proportional to the number of viable cells in the sample. The fluorometer is portable. The device was tested with both lyophilized and fresh whole blood serums that were spiked with E.coli. Concentrations as low as 10 CFU/mL were detected. This paper discusses both the procedure to detect the bacteria as well as the results for different bacterial concentrations.","PeriodicalId":120815,"journal":{"name":"2017 IEEE Healthcare Innovations and Point of Care Technologies (HI-POCT)","volume":"2 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"A highly sensitive microfluidic device for bacterial detection in blood serum\",\"authors\":\"M. Al-Adhami, E. Tan, G. Rao, Y. Rostov\",\"doi\":\"10.1109/HIC.2017.8227601\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Highly sensitive device to detect bacteria in blood serum is presented. The device comprises of a microfluidic component and an electronic reader. The microfluidic cassette acts as an enclosed vial. It is filled with the sample after mixing with an indicator dye. Then, it is inserted into a kinetics fluorometer. The rate of the fluorescence increase is proportional to the number of viable cells in the sample. The fluorometer is portable. The device was tested with both lyophilized and fresh whole blood serums that were spiked with E.coli. Concentrations as low as 10 CFU/mL were detected. This paper discusses both the procedure to detect the bacteria as well as the results for different bacterial concentrations.\",\"PeriodicalId\":120815,\"journal\":{\"name\":\"2017 IEEE Healthcare Innovations and Point of Care Technologies (HI-POCT)\",\"volume\":\"2 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2017 IEEE Healthcare Innovations and Point of Care Technologies (HI-POCT)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/HIC.2017.8227601\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2017 IEEE Healthcare Innovations and Point of Care Technologies (HI-POCT)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/HIC.2017.8227601","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A highly sensitive microfluidic device for bacterial detection in blood serum
Highly sensitive device to detect bacteria in blood serum is presented. The device comprises of a microfluidic component and an electronic reader. The microfluidic cassette acts as an enclosed vial. It is filled with the sample after mixing with an indicator dye. Then, it is inserted into a kinetics fluorometer. The rate of the fluorescence increase is proportional to the number of viable cells in the sample. The fluorometer is portable. The device was tested with both lyophilized and fresh whole blood serums that were spiked with E.coli. Concentrations as low as 10 CFU/mL were detected. This paper discusses both the procedure to detect the bacteria as well as the results for different bacterial concentrations.
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