三种柑桔果皮精油抑菌作用的比较研究

Edogbanya Pro, Suleiman Mo, Olorunmola Jb, Oijagbe Ij
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引用次数: 19

摘要

多年来,为了找到处理对传统抗生素产生耐药性的细菌菌株的替代方法,使用精油作为抗菌剂已经变得很流行。本研究比较了产自尼日利亚科吉州Okene Main Market(7’33’4.39”N 6’14’9.20”E)的柑橘皮精油对临床分离的几种微生物(大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和黑曲霉)的抗菌作用。以正己烷为溶剂,采用冷浸法提取果皮中的油脂。采用琼脂扩散法,以环丙沙星为标准阳性对照,对菌株进行药敏试验。试验采用重复进行,所得数据采用单因素方差分析(ANOVA)和Duncan多元极差检验(DMRT)进行分析,以P<0.05为显著性。结果表明,柑桔(Citrus sinensis)对试验菌株的抑制作用最大,其次是酸橙(Citus aurantifolia)和柠檬(Citrus Limon),其抑制作用最不显著。
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Comparative study on the antimicrobial effects of essential oils from peels of three citrus fruits
The use of Essential Oils as antimicrobial agents have become popular over the years in an attempt to find alternative ways of dealing with strains of bacteria that have become resistant to conventional antibiotics. This study was carried out to compare the antimicrobial effects of Citrus peel essential oils obtained from Okene Main Market, 7'33'4.39'' N 6'14'9.20'' E, Kogi State, Nigeria, on the clinical isolates of some microorganisms (Escherichia coli, Pseudesomonas aeruginosa, Staphylococcus aureus, and Aspergillus niger). The oils were extracted from the peels using the cold maceration method with n-hexane as the solvent. The agar diffusion method was used to test the susceptibility of the micro-organism strains using ciprofloxacin as the standard positive control. The experiment was carried out in duplicates and obtained data was analysed using one-way analysis of variance (ANOVA) and Duncan Multiple Range Test (DMRT), with P<0.05 considered significant. The results revealed that Orange (Citrus sinensis) exhibited the inhibitoriest effect on the test isolates followed by lime (Citus aurantifolia) and Lemon (Citrus Limon) with the least significant effect.
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