用纤维素酶酶解从香蕉皮(Musa paradisiaca L.)中提取葡萄糖糖浆

Alicia Decheco Egúsquiza
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摘要

目的是确定真菌纤维素酶对香蕉丝皮(Musa paradisiaca)制备葡萄糖糖浆的影响。在50℃恒温条件下,研究了30% (w / v)香蕉壳的酶解,控制的自变量为真菌纤维素酶的浓度(0.5%、1%和1.5% w / v)和酶解时间(18、24和30 h)。因变量为酶解样品中还原糖的百分比和葡萄糖当量的百分比(D.E.)。试验设计完全随机化,采用因子模型32。采用真菌纤维素酶水解丝车前草壳制备葡萄糖糖浆。当纤维素酶浓度为1.5% (w / v)时,水解时间为30小时,还原糖和葡萄糖当量的百分比最高,平均还原糖百分比为15.5%,葡萄糖当量百分比为30.9%。用真菌纤维素酶在1.5% (w / v)条件下水解30小时,获得了高附加值的产品,确定了以香蕉皮为原料生产葡萄糖糖浆的操作条件和工艺步骤。
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Obtención de jarabes glucosados a partir de cáscaras de Plátano (Musa paradisiaca L.) mediante hidrólisis enzimática de celulasas
The objective was to determine the effect of the application of the fungal cellulase enzyme on the silk banana peels (Musa paradisiaca) to obtain glucosed syrup. The enzymatic hydrolysis of banana shells at 30% (w / v) was studied at a constant temperature of 50 ° C. The independent variables to be controlled were the concentration of the fungal cellulase enzyme (0.5%, 1% and 1.5% w / v) and the hydrolysis time (18, 24 and 30 hours). The dependent variables were the percentage of Reducer Sugar and the percentage of Dextrose Equivalent (D.E.) of the enzymatically hydrolysed samples. The experimental design was completely randomized with factorial model 32. Glucosed syrups were obtained from silk plantain hulls by the enzymatic hydrolysis of the fungal cellulase. The highest percentages of Reducer Sugar and Dextrose Equivalent were presented in the Cellulase Concentration at 1.5% (w / v) during a hydrolysis time of 30 hours with average values ​​of 15.5% of reducing sugar percentage and 30.9% of percentage of Dextrose Equivalent. In the enzymatic hydrolysis with fungal cellulase at 1.5% (w / v) and 30 hours a higher value-added product was obtained, the operating conditions and the process steps were defined that should be adapted for the production of glucosed syrup from of banana peels (Musa paradisiaca).
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