香蕉卡文迪许的体外培养(摩西acuminata L)不同浓度的介质中提取番石榴和氨基酸苯二胺

Adam Saepudin, Amir Amilin, Undang Undang, Tini Sudartini
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摘要

体外培养需要合适的培养基,添加BAP作为细胞分裂素被认为可以促进卡文迪什香蕉的生长。在香蕉外植体的生长中也经常发现外植体褐变的限制。番石榴提取物作为有机抗氧化剂和BAP的添加有望抑制卡文迪什香蕉外植体的褐变,促进其体外生长。本研究旨在确定番石榴提取物(Psidium guajava)和苄基氨基嘌呤(Benzyl Amino Purine)在卡文迪什香蕉(Musa acuminata)离体培养中的最佳浓度。本研究采用完全随机设计(CRD),阶乘模式为4 × 3,重复4次。处理时在培养基中分别添加番石榴提取物(0 g/l、1 g/l、2 g/l、3 g/l)和BAP (3 ppm、6 ppm、9 ppm)。对数据进行方差分析,采用F检验,继续采用Duncan多重距离检验,显著性水平为5%。结果表明:1 g/l番石榴浸膏+ BAP 3 ppm处理的芽数最好;以0 g/l番石榴浸膏+ BAP 6 ppm处理的叶片数和高芽数效果最好
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Kultur In Vitro Pisang Cavendish (Musa acuminata L.) Pada Media Dengan Konsentrasi Berbeda Ekstrak Jambu Batu Dan Benzyl Amino Purine
In vitro culture requires suitable media, the addition of BAP as a cytokinin is considered to accelerate the growth of Cavendish bananas. Constraints of browning in explants are also often found in the growth of banana explants. The addition of guava extract as an organic antioxidant and BAP is expected to suppress browning and improve the growth of Cavendish banana explants in vitro. The study was aimed to obtain the best concentration of guava extract (Psidium guajava) and Benzyl Amino Purine for the growth of Cavendish banana (Musa acuminata) explants in vitro. This study used a completely randomized design (CRD) with a factorial pattern of 4 x 3 with 4 replications. Treatment of guava extract added to the media (0 g/l, 1 g/l, 2 g/l, 3 g/l) and BAP (3 ppm, 6 ppm, 9 ppm). The data were analyzed using variance with the F test and continued with Duncan's Multiple Distance Test with 5% significance level. The results showed that the best result for the number of shoots were in the treatment of 1 g/l guava extract + BAP 3 ppm. In the number of leaves and high shoots the best result were in the treatment of 0 g/l guava extract + BAP 6 ppm
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