果蝇Om突变体的细胞学定位。

Idengaku zasshi Pub Date : 1992-06-01 DOI:10.1266/jjg.67.259
H Matsubayashi, M Matsuda, Y Tomimura, M Shibata, Y N Tobari
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引用次数: 10

摘要

果蝇的半显性、光学形态(Om)突变已被定位到整个基因组中至少25个位点(Hinton, 1984;1988)。其中,4个x连锁的Om突变体被证明与转座因子的插入有关,tom (Shrimpton et al., 1986;Tanda et al., 1988)。在本研究中,利用克隆的tom元件作为探针,通过原位杂交对多烯染色体进行常染色体Om突变体的细胞学定位。每个常染色体Om突变体的细胞学位点已确定为唾液腺染色体的单个带。
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Cytological mapping of Om mutants of Drosophila ananassae.

Semidominant, optic morphology (Om) mutants in Drosophila ananassae have been genetically mapped to at least 25 loci throughout the genome (Hinton, 1984; 1988). Among them, four X-linked Om mutants were proved to be associated with the insertion of a transposable element, tom (Shrimpton et al., 1986; Tanda et al., 1988). In the present study, cytological mapping of autosomal Om mutants was carried out by in situ hybridization to polytene chromosomes using a cloned tom element as a probe. The cytological site for each autosomal Om mutant has been determined to a single band of the salivary gland chromosomes.

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