米巴棕榈叶乙醇提取物(Azadirachta indica A. Juss)对白鼠肝病理结构的影响(Rattus norvegicus L)。雄性血糖过高

Arthi Ridho Wicaksono, Siti Muflichatun Mardiati, Sri Isdadiyanto
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引用次数: 0

摘要

高血糖是一种高血糖的疾病。高血糖会导致糖尿病,破坏hepar组织的组织结构。Mimba (Azadirachta indica A.Juss)是一种生长在印度尼西亚不同地区的草药。米巴叶乙醇提取物含有抗氧化剂黄酮,可以帮助修复受损的hepar组织。本研究的目的是分析菌丝叶乙醇提取物对修复hepar结构的影响。这项研究采用了完全随机设计。白鼠(学名Rattus norvegicus L.)共18只,可分为6种治疗方法和3种循环。这项研究进行了27天。老鼠的正常情况被标记为水貂。老鼠的高血糖状态得到控制。小鼠的多糖神经增生剂量为2.25毫克/kg。P3、P4和P5是一组高血糖小鼠,每次剂量100、200和400毫克BB。小鼠的高血糖状况是由120毫克/kgBw剂量的生发动物引起的。研究数据以95%的显著性为基础进行分析,而未正常分布的数据则通过Kruskal-Wallis测试进行分析。结果表明,米巴叶乙醇提取物对病原直径参数和病原损伤量表(p0.05)有明显的影响。这项研究的结论是,米巴的叶子乙醇提取物(Azadirachta indica A. gbb)的剂量为雄性白斑小鼠组织提供了有效的保护,使其免受因诱发高血糖而引起的高血糖状态的高血糖。高血糖通常导致已知的糖尿病能够破坏组织组织的组织结构。Neem (Azadirachta inca .Juss)是一个在不同地区常见的药用植物。细亚奥利亚的排他性叶接触抗氧化剂类黄糖,可以帮助修复受损肝脏组织组织。这项研究的目标是分析尼姆对受损肝脏组织的治疗特性。这项研究用的是一个完整的分级设计模型,由18只白色老鼠组成,分布在6个小组和3个区域。研究还停留了27天。一群老鼠在正常情况下会发出少量的水。P1(消极控制)P2(阳性控制)P3、P4和P5是由一群以100,200和400 mg/Kg为单位提供的高血糖条件的老鼠组成的。老鼠的高血糖状况是代诺州批准了120毫克/kgBw。研究的结果是用95%的意味价进行样本分析。对于不正常分配的数据,Kruskal-Wallis测试是用来分析数据的。结果表明,尼姆的政府留下了一种对病变直径及其scale的严重影响(p0.05)。这项研究的结论是,ethanolic提出了400毫克/公斤的身体重量,可以保护和恢复由不同诱导的白老鼠产生的损害。
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Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) Terhadap Struktur Histopatologi Hepar Tikus Putih (Rattus norvegicus L.) Jantan Hiperglikemia
Hiperglikemia merupakan kondisi medis yang diindikasikan dengan tingginya kadar glukosa darah. Hiperglikemia dapat menyebabkan kondisi diabetes yang dapat merusak struktur histologis jaringan hepar. Mimba (Azadirachta indica A.Juss) merupakan tumbuhan obat yang dikenal di berbagai daerah di Indonesia. Ekstrak etanol daun mimba mengandung antioksidan flavonoid yang dapat membantu dalam perbaikan jaringan hepar yang rusak. Penelitian ini bertujuan untuk menganalisis efek ekstrak etanol daun mimba dalam perbaikan struktur histologis hepar. Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL). Tikus putih (Rattus norvegicus L.) sebanyak 18 ekor dibagi menjadi 6 kelompok perlakuan dan 3 kali perulangan. Penelitian dilaksanakan selama 27 hari. P0 (kontrol normal) tikus kondisi normal diberi akuades. P1 (kontrol negatif) tikus kondisi hiperglikemi diberi akuades. P2 (kontrol positif) tikus kondisi hiperglikemi diberi glibenklamid dosis 2.25 mg/kg BB. P3, P4, dan P5 merupakan kelompok tikus hiperglikemi yang diberi perlakuan ekstrak etanol daun mimba dengan dosis masing-masing 100, 200, dan 400 mg/kg BB. Kondisi hiperglikemi tikus diinduksi dengan pemberian aloksan dengan dosis 120 mg/kgBw. Data penelitian dianalisis menggunakan uji Anova pada signifikansi 95%, sedangkan data yang tidak terdistribusi secara normal dianalisis dengan uji Kruskal-Wallis. Hasil menunjukkan bahwa pemberian ekstrak etanol daun mimba berpengaruh nyata pada parameter diameter hepatosit dan skala kerusakan hepatosit (P<0,05), namun tidak memberikan pengaruh nyata pada parameter hepatosomatik indeks (P>0,05). Kesimpulan penelitian ini adalah pemberian ekstrak etanol daun mimba (Azadirachta indica A.Juss) dosis 400mg/KgBB efektif melindungi jaringan hepar tikus putih jantan dari kondisi hiperglikemia akibat induksi aloksan  Hyperglycemia is a medical condition indicated by the abnormally high blood sugar level in one’s body. Hyperglycemia usually leads to diabetes which is known to be capable of damaging the histological structures of liver tissues. Neem (Azadirachta indica A.Juss) is a well-known medicinal plant in various regions. Ethanolic extract of neem leaves contains the antioxidant flavonoid that can help repair damaged liver tissues. The aim of this research was to analyze the healing properties of the neem leaves ethanol extracts to the damaged liver tissues. This research used the Completely Randomized Design (CRD) model with a total of 18 male white rats which were divided into 6 treatment groups and 3 iterations. The research was carried out for 27 days. P0 (Normal control) the group of rats in normal condition were given distilled water. P1 (negative control) the group of rats in hyperglycemic condition were given distilled water. P2 (Positive control) the group of rats in hyperglycemic condition were given glibenclamide with the dose of 2.25 mg/kg Body Weight. P3, P4, and P5 were the group of rats in hyperglycemic conditions who were given neem leaves ethanol extract with doses each 100, 200, and 400 mg/Kg Body Weight. The hyperglycemic condition in rats was induced by administration of 120 mg/kgBw of alloxan. The findings from the research were analyzed using the Anova test at a 95% significance rate. As for the data that was not normally distributed, the Kruskal-Wallis test was used to analyze the dataset. The results showed that the administration of neem leaves ethanol extract had a significant effect on the parameters of hepatocyte diameter and the scale of hepatocyte damage (P<0.05), but did not significantly affect the parameter of the hepatosomatic index (P>0.05). The conclusion of this study was that the ethanolic extract of neem leaves with the dose of 400 mg/Kg body weight can indeed effectively protect and repair the damages induced by the alloxan-induced hyperglycemia in male white rats.
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