Stress causing dynamic changes of four phytohormones in tobacco and tomato: A GC-MS analysis

Many analytical methods are in use to analyse plant hormones in different plants. Here this work provides a sensitive, accurate and quite accessible GC-MS (gas chromatography/mass spectrometry) method to quantify phytohormones indole-3-aceticacid (IAA), abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). These signaling molecules were analysed in two different plants, tomato and tobacco grown in vitro. The protocol designed to assess, how abiotic stress brings about changes in the level of endogenous hormones in the leaves of both the plants under study. A hormone profile of salt stressed leaves shows that different plant hormones are involved in diverse physiologicsl processes. Crosstalk between these hormones result in synergetic or antagonic interactions which have important roles to play in abiotic stress response.