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Application of Chemometrics and HPLC Fingerprint for Species Differentiation and Authentication of the Genus Pterocarpus 化学计量学和HPLC指纹图谱在翼果属植物种类鉴别和鉴定中的应用
Pub Date : 2023-05-30 DOI: 10.25081/ripb.2023.v13.8292
T. K. Muftaudeen, A. Aliyu, J. Ibrahim
Pterocarpus species are well known for their nutritional and medicinal values, in order to examine the similarities or differences in the chemical profile of some common Pterocarpus species, four species of the genus namely; Pterocarpus erinaceus, P. mildbraedii, P. osun and P. santalinoides were analyzed using HPLC combined with Principal component analysis (PCA) and Hierarchical clustering analysis (HCA). This study aims to investigate the chemical fingerprints of the species and compare them in order to highlight the similarities in their chemical constituents. The ethanol extract of each sample was taken and filtered through a 0.45 μm millipore membrane filter and then transferred into the HPLC vial before injecting it into the HPLC machine. PCA and HCA were performed on the relative retention times and percentage peak composition. The species were chemically similar with nine (9) peaks in common, the most prominent peaks in all samples appeared at 3.26 min which corresponds to gallic acid (a known compound). Cluster analysis revealed some similar chemical variables with Gallic acid being the major compound in the Pterocarpus species and could be used as a marker compound for taxonomic purposes.
蕨类植物以其营养价值和药用价值而闻名,为了研究一些常见的蕨类植物在化学特征上的异同,本属的四种即;采用高效液相色谱法(HPLC)结合主成分分析(PCA)和层次聚类分析(HCA)对羊角蕨(Pterocarpus erinaceus)、马尾蕨(P. mildbraedii)、羊尾蕨(P. osun)和桑塔林蕨(P. santalinoides)进行分析。本研究旨在研究该物种的化学指纹图谱,并对其进行比较,以突出其化学成分的相似性。取每个样品的乙醇提取物,经0.45 μm毫孔膜过滤器过滤后,转移到HPLC瓶中,再注射到HPLC机中。采用PCA和HCA对相对保留时间和峰组成百分比进行分析。所有样品在3.26 min出现最显著的峰,对应于没食子酸(一种已知化合物)。聚类分析结果表明,该属植物的主要化学成分为没食子酸,可作为分类的标记化合物。
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引用次数: 0
Foliar epidermal studies in the family Sapindaceae Juss. in South Western Nigeria 标题皂荚科植物叶面表皮的研究。在尼日利亚西南部
Pub Date : 2023-03-08 DOI: 10.25081/ripb.2023.v13.8199
T. K. Muftaudeen, M. Bulama, E. Ayodele, A. Akinloye
A comparative study of leaf epidermal features of selected species of the family Sapindaceae in Nigeria was undertaken with a view to obtain additional characters for the elucidation of taxonomic relationships, delimiting species and identification of the taxa. The epidermises were separated from the mesophyll by soaking in concentrated Nitric acid. Bubble appearance on the surfaces of the leaves indicated their readiness for separation. They were then rinsed in water thrice before the layers were separated with the aid of forceps and Camel hair brush, stained with Safranin O. It was then mounted in 25% glycerol on clean glass slides, covered with cover slips. The micro morphological characters of leaf were more or less uniform in all taxa in the family. The adaxial cuticle was striated in most species. The polygonal epidermal cells with straight anticlinal walls on the adaxial surface of Cardiosermum halicacabum separated it from the other species with irregular shaped epidermal cells. Undulated anticlinal walls were observed on both surfaces. The leaves were hypostomatic in most species except in C. halicacabum where amphistomaty was observed. Stomata types included anomocytic, tetracytic and staurocytic but the anomocytic stomata were the most predominant.
摘要对尼日利亚皂荚科植物的叶表皮特征进行了比较研究,以期获得更多的特征,用于分类关系的阐明、种间的划分和分类群的鉴定。用浓硝酸浸泡将表皮与叶肉分离。叶子表面的气泡表明它们准备好分离了。然后用水冲洗三次,然后用镊子和骆驼毛刷分离,用红花红o染色,然后用25%甘油装在干净的玻璃载玻片上,盖上盖片。本科各分类群叶片微形态特征基本一致。大多数种的正面角质层呈条纹状。halicacabum的正面表皮细胞呈多角形,背斜壁直,与其他种类表皮细胞呈不规则形状的植物不同。两表面均可见波浪形背斜壁。大多数种类的叶片都是低气孔的,除了在海棠花中观察到两形气孔。气孔类型包括不规则型、四胞型和小细胞型,但以不规则型气孔为主。
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引用次数: 0
Morpho-agronomic variability, traits association and path analysis in Rosemary (Salvia rosmarinus Schleid.) genotypes from Ethiopia 埃塞俄比亚迷迭香基因型的形态农艺变异、性状关联及通径分析
Pub Date : 2023-02-17 DOI: 10.25081/ripb.2023.v13.8128
Zewdinesh Damtew Zigene, Bizuayehu Tesfaye Asfaw, Tesfaye Disasa Bitima
Forty-five rosemary genotypes collected from different parts of Ethiopia were evaluated for quantitative morphological traits to estimate the genetic variability, heritability and association of characters. The genotypes displayed significant differences for all of the studied traits, showing the presence of wide variability among the tested genotypes. The genotypic and phenotypic coefficients of variation were found to be medium and high for all growth and yield traits except for leaf length. Moderately high to high broad-sense heritability (0.66 -0.97) coupled with moderate to high genetic advance as a percent of the mean (10.37 -79.14) values were found for all traits.  Correlation coefficient and path coefficient analysis revealed that characters vis. branch number plant-1, fresh leaf weight plant-1, dry leaf weight plant-1, fresh leaf yield ha-1, and essential oil content had a high positive direct effect and significant positive association with essential oil yield ha-1. Thus direct selection for these traits would be quite effective for essential oil yield enhancement in rosemary. In general, the studied accessions were diverse in nature and could be exploited in the conservation, breeding and commercialization of the crop.
对采自埃塞俄比亚不同地区的45个迷迭香基因型进行了数量形态性状评价,以估计遗传变异、遗传力和性状关联。基因型在所有性状上均表现出显著差异,表明在被测基因型之间存在广泛的变异性。除叶长外,所有生长性状和产量性状的基因型和表型变异系数均为中、高。所有性状的广义遗传力均为中高至高(0.66 ~ 0.97),遗传进步占平均值的百分比为中高至高(10.37 ~ 79.14)。相关系数和通径分析结果表明,枝数plant-1、鲜叶重plant-1、干叶重plant-1、鲜叶产量ha-1、挥发油含量等性状对挥发油产量ha-1有较高的正直接影响,且与挥发油产量ha-1呈显著正相关。因此,直接选择这些性状对提高迷迭香精油产量是非常有效的。总的来说,所研究的材料具有多样性,可用于该作物的保护、育种和商业化。
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引用次数: 0
Leaf and fruit characteristics of Parkia biglobosa (Jacq.) Benth according to agro climatic zones and land use in Southern Mali 枇杷叶和果实的特性根据马里南部的农业气候带和土地利用
Pub Date : 2022-02-22 DOI: 10.25081/ripb.2022.v12.7238
B. Kelly, A. Kouyaté, S. Dembélé
A study was conducted in southern Mali to characterized Parkia biglobosa populations in the aim to contribute to the domestication of the species. Fields and fallows in the north sudanian, south sudanian and north guinean zones were concerned. Adult trees were marked in plots of 0.25 ha each and leaves and fruits were harvested from each tree for characterization. Petiole and laminar length, number of pairs of pinnae and leaflets, pedicel and pod length, pod wide, pod and seed weight, number of seed per pod were measured. The north guinean zone showed the highest number of pairs of pinnae and leaflets, the longest pods (23.06±9.91 cm in the fields), the highest mean seed weight (3.74±0.96 g in the fields) and the highest mean number of seeds per pod (17 ± 3 seeds in the fields). The South Sudanian zone showed the longest pedicels (3.85±1.08 cm in the fallows), the highest mean laminar length (18.02 cm) and mean petiole length (6.45 cm) in the fields but the same mean number of seeds per pod as for the north Guinean. The north Sudanian zone showed the widest (17.88±7.98 cm in the fields and 18.24±2.11 cm in the fallows) and heaviest (14.87±3.95 g in the fallows) pods. Leaf variables like the number of pairs of pinnae and leaflets seem to be influenced by the climatic gradient. The others leaf, pod and seed variables seem not to be influenced by the climatic gradient. These findings suggest that many leaf and fruit variables are controlled by endogenous factor that could be genetic. However, the manifestation of the endogenous factor seems to be influenced by factors such as environment and management practices.
在马里南部进行了一项研究,以表征大白斑鹦鹉种群,目的是促进该物种的驯化。所涉及的是北苏丹、南苏丹和北几内亚地区的田地和休耕地。成树在每块0.25 ha的地块上进行标记,并从每棵树上收获叶子和果实进行鉴定。测定了叶柄和层长、羽片和小叶对数、花梗和荚果长、荚果宽、荚果和种子重、每荚果种子数。北几内亚区荚果最长(田间为23.06±9.91 cm),荚果平均重最高(田间为3.74±0.96 g),荚果平均粒数最高(田间为17±3粒)。南苏丹地区的花梗最长(根部3.85±1.08 cm),平均层长(18.02 cm)和平均叶柄长(6.45 cm),但每荚的平均种子数与北几内亚地区相同。北苏丹区豆荚最宽(田间17.88±7.98 cm,休耕地18.24±2.11 cm),最重(休耕地14.87±3.95 g)。像羽片和小叶的成对数这样的叶片变量似乎受到气候梯度的影响。其他叶、荚和种子变量似乎不受气候梯度的影响。这些发现表明,许多叶片和果实的变量是由可能是遗传的内源因素控制的。然而,内生因素的表现似乎受到环境和管理实践等因素的影响。
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引用次数: 0
Wood anatomical features of some Nigerian species of Acacia Mill and their suitability for paper making 尼日利亚一些金合欢树种的木材解剖特征及其造纸适宜性
Pub Date : 2021-12-30 DOI: 10.25081/ripb.2021.v11.6517
S. M. Owolabi, Monday Daniel Odiye, A. Akinloye, A. Ayodele
A comparative wood anatomical study of six species of the genus Acacia commonly found in Nigeria was carried out. Wood samples were collected from matured branches of plants in different locations in Mazah and Shere Hills, Jos, Plateau State, Nigeria. Transverse, tangential and radial longitudinal sections as well as wood macerates were prepared and mounted unto microscopic slides using standard anatomical methods. All the species had diffused porous wood, heterogeneous rays, aseptate fibres and predominantly solitary vessels with simple perforation plate, few pore clusters and pore multiples. Banded paratracheal axial parenchyma was common in all taxa except in A. senegal which was predominantly paratracheal without bands. Multiseriate rays were common features in all taxa except in A. nilotica which had predominantly uniseriate rays. Quantitative wood anatomical characters such as fibre length, fibre diameter, fibre lumen diameter, fibre wall thickness, vessel length, vessel diameter, ray length, ray diameter and pore diameter were of taxonomic importance in the delimitation of the studied taxa. The Runkel ratio of A. senegal (0.99µm) compared favourably with some hardwood species in the Nigerian rainforest ecosystem hence this taxon could be exploited for pulp and paper in Nigeria.
对尼日利亚常见的金合欢属6种进行了木材解剖比较研究。木材样本是从尼日利亚乔斯高原州Mazah和Shere Hills不同地点的植物成熟树枝上收集的。横向,切向和径向纵剖面以及木浸渍准备和安装到显微镜载玻片使用标准的解剖方法。所有树种均具有弥散多孔木材、非均质射线、分离纤维和以单生血管为主,孔板简单,孔簇和孔倍较少。带状的气管旁轴向薄壁在所有类群中都很常见,除了在塞内加尔沙蚤中以无带状的气管旁为主。除nilotica以单列射线为主外,其他类群均具有多列射线特征。木材的纤维长度、纤维直径、纤维管腔直径、纤维壁厚、血管长度、血管直径、射线长度、射线直径和孔径等定量解剖特征对研究分类群的划分具有重要的分类意义。该分类群的Runkel比值(0.99µm)优于尼日利亚热带雨林生态系统中的一些硬木物种,因此该分类群可用于尼日利亚的纸浆和造纸。
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引用次数: 1
Characterization of non polar-polar solvent extracts from some tea plantation shade tree leaves with special reference to antioxidant and antibacterial activities 部分茶园遮荫树叶片非极性溶剂提取物的表征及其抗氧化和抗菌活性
Pub Date : 2020-09-01 DOI: 10.25081/ripb.2020.v10.6235
A. Ghosh, Soumya Majumder, S. Saha, M. Bhattacharya
Beneficial properties of shade trees of tea plantations other than their medicinal properties have been extensively studied. This research was initiated to explore the properties of some shade trees with special emphasis on their antioxidant and antibacterial properties. Leaves from shade tree like Dalbergia sissoo (DS), Cassia siamea (CS), Derris robusta (DR), Leucaena leucocephala (LL), Acacia lenticularis (AL) and Melia azedarach (MA) were used for the study. Characterization of shade tree leaves by determination of moisture, crude fibre and ash content and tests of non polar – polar solvent extracts for steroid, tannins, cardiac glycosides and coumarin, free radical scavenging, ferric reducing power, NO scavenging activities, quantification of Flavonoids and antibacterial activity were conducted. The average moisture, crude fibre and ash percentage of shade tree plants were found to be 62.95, 11.28 and 1.86 respectively. Methanol, ethanol, acetone and ethyl acetate respectively proved to be the most potent solvent for various phytochemical extractions as it gave positive results for tests like tannin, steroid, cardiac glycosides and coumarin. AL (91.46%), DR (92.69%), LL (94.32%) and MA (93.34%) leaf extracts showed a high level of DPPH scavenging activity in their water extracts. In DS (88.11%) and CS (83.23%) maximum DPPH scavenging activity was observed in Diethyl ether and Methanol extracts respectively.  Acetone extracts were more active than the water extracts in exhibiting ferric reducing power and NO scavenging activity. Summation of the quantity revealed that DS showed maximum presence of flavonoids and acetone as most potential for isolation of flavonoids. The decreasing order of summative antibacterial activity was recorded in DS, followed by CS, DR, AL, MA and LL. Chloroform showed the highest summative inhibition zone followed by ethanol, ethyl acetate, diethyl ether, acetone, water,  hexane, benzene and methanol. The antioxidant and antibacterial potential of shade trees were established.
除药用外,茶园遮荫树的有益特性已被广泛研究。本研究旨在探讨一些遮荫树的特性,重点研究其抗氧化和抗菌特性。以黄檀(DS)、桂花(CS)、苦楝(DR)、银合欢(LL)、金合欢(AL)和苦楝(MA)等遮荫树种的叶片为研究对象。通过水分、粗纤维和灰分含量的测定对遮荫树叶片进行了表征,并对非极性-极性溶剂提取物的甾体、单宁、心糖苷和香豆素、自由基清除能力、铁还原能力、NO清除能力、黄酮类化合物的定量和抗菌活性进行了测试。遮荫乔木的平均水分、粗纤维和灰分率分别为62.95、11.28和1.86。甲醇、乙醇、丙酮和乙酸乙酯分别被证明是各种植物化学提取的最有效溶剂,因为它对单宁、类固醇、心糖苷和香豆素等测试都有阳性结果。AL(91.46%)、DR(92.69%)、LL(94.32%)和MA(93.34%)叶提取物对DPPH的清除活性较高。在DS(88.11%)和CS(83.23%)中,乙醚和甲醇提取物对DPPH的清除作用最大。丙酮提取物的铁还原能力和NO清除能力均优于水提取物。结果表明,DS中黄酮类化合物含量最高,丙酮类化合物最有分离潜力。DS的总抗菌活性依次为CS、DR、AL、MA和LL。氯仿的总抑制区最大,其次是乙醇、乙酸乙酯、乙醚、丙酮、水、己烷、苯和甲醇。确定了遮荫树的抗氧化和抗菌潜力。
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引用次数: 1
Genetic variability and regression analysis for yield and quality traits in Brassica napus 甘蓝型油菜产量和品质性状的遗传变异及回归分析
Pub Date : 2020-08-08 DOI: 10.25081/ripb.2020.v10.6287
Nabeel Khan, Raziuddin, N. Ahmad, Fazli Ahad
An experiment was conducted to estimate the genetic variability and regression analysis for yield and quality traits in a set of eight brassica genotypes in a randomized complete block design with three replications at The University of Agriculture Peshawar, during 2011-12 cropping season. Significant genetic variability was recorded among genotypes for plant height, pods main receme-1, pod length, seed yield plant-1, 1000-seed weight, seeds pod-1, oil content, protein content, glucosinolate content, erucic acid content, oleic acid content and linolenic acid content except for primary branch plant-1 and pods plant-1 which showed non-significant differences. Genotypes, AUP-401, AUP-402, AUP-404 and AUP-407 showed potential performance for glucosinolate content (55.1 μmol g-1), seed yield plant-1 (23.93 g), pods plant-1 (268.83), protein contents (21.1 %), erucic acid contents (42.7 %) and oil contents (53.3 %). Seed yield plant-1 was taken as a dependent variable to study the effects of many morphological yield traits on it. Branches plant-1, pods main raceme-1, pods plant-1, and seed pod-1 showed a positive effect on seed yield plant-1, while 1000-seed weight, pod length, and plant height showed a negative effect on seed yield plant-1. Seed yield plant-1 as an independent variable showed a negative effect on oil content. Protein content as an independent variable showed a positive effect on erucic acid content, while oil contents as independent variables show a negative effect on protein contents, respectively. The developed germplasm could be used in the upcoming brassica breeding programs to release high yielding, disease resistance, stress tolerance, and insect resistance varieties, which will eventually upsurge the productivity and decrease the gap between demand and domestic production of the brassica crop.
2011- 2012年种植季,在白沙瓦农业大学采用3个重复的随机完全区组设计,对8个芸芥基因型进行了产量和品质性状的遗传变异估计和回归分析。株高、荚果主receme-1、荚果长、种子产量plant-1、千粒重、种子pod-1、含油量、蛋白质含量、硫代葡萄糖苷含量、芥酸含量、油酸含量和亚麻酸含量等基因型间存在显著遗传变异,但初级分枝植物-1和荚果植物-1差异不显著。基因型AUP-401、AUP-402、AUP-404和AUP-407在硫代葡萄糖苷含量(55.1 μmol g-1)、籽粒产量(23.93 g)、荚果产量(268.83 g)、蛋白质含量(21.1%)、芥酸含量(42.7%)和油含量(53.3%)方面表现出潜在的性能。以种子产量植物1号为因变量,研究了多种形态性状对其产量的影响。分枝植物-1、荚果总状花序-1、荚果植物-1和种子荚-1对种子产量有正向影响,而千粒重、荚果长和株高对种子产量有负向影响。种子产量plant-1作为自变量对含油量呈负相关。以蛋白质含量为自变量对芥酸含量有正影响,以油脂含量为自变量对蛋白质含量有负影响。所开发的种质资源可用于未来的芸苔育种计划,以推出高产、抗病、抗逆性和抗虫性品种,最终提高芸苔产量,缩小需求与国内产量之间的差距。
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引用次数: 1
Variability for agromorphological traits, genetic parameters, correlation and path coefficient analyses in Lentil (Lens culinaris Medik.) 小扁豆(Lens culinaris Medik.)农态性状变异、遗传参数、相关及通径分析
Pub Date : 2020-07-03 DOI: 10.25081/ripb.2020.v10.6237
S. Akter, I. Jahan, Md. Amir Hossain, M. A. Hossain
Lentil is (Lens culinaris Medik.) also popularly known as Masur which is extensively grown in Bangladesh for its economic importance. It is the second most important pulse crop both in acreage and production but stands first in consumer’s preference in Bangladesh [1]. Lentil is considered as poor mean’s meat, a substitute of animal protein for the underprivileged people of Bangladesh who cannot afford to buy animal protein [2,3]. It is an excellent source of carbohydrates, vitamins, minerals and several essential micro-nutrients like iron, zinc and betacarotene [4,5,6]. The crop has great significance in cereal-based cropping systems because it can fix atmospheric nitrogen and thereby enriching the soil by Rhizobium bacteria [7,8]. Recently, lentil has received considerable interest by the scientific community due to its higher nutritive value and lower seed yield plant-1. Increasing yield is therefore the prime breeding objectives of lentil breeder around the globe including Bangladesh. Low production and acreage of lentil in Bangladesh is due to the shortage of lentil varieties with high yield, poor technological intervention during production and competition for a place in the existing cropping pattern. This lacking can be overcome by developing high yielding varieties with wider adaptability and stability.
小扁豆(Lens culinaris Medik.)也被普遍称为Masur,因其经济重要性而在孟加拉国广泛种植。在种植面积和产量方面,它是第二大重要的豆类作物,但在孟加拉国,消费者的偏好排名第一[1]。扁豆被认为是穷人的肉,是孟加拉那些买不起动物蛋白的贫困人群的动物蛋白替代品[2,3]。它是碳水化合物、维生素、矿物质和几种必需微量营养素(如铁、锌和β胡萝卜素)的极好来源[4,5,6]。该作物在以谷物为基础的种植系统中具有重要意义,因为它可以固定大气氮,从而通过根瘤菌丰富土壤[7,8]。近年来,小扁豆因其营养价值高、种子产量低的特点受到了科学界的广泛关注。因此,提高产量是包括孟加拉国在内的全球扁豆育种者的主要育种目标。孟加拉国扁豆产量和种植面积低的原因是缺乏高产扁豆品种,生产过程中技术干预不足,以及在现有种植模式中争夺一席之地。这一缺陷可以通过开发适应性和稳定性更强的高产品种来克服。
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引用次数: 2
Micropropagation, genetic fidelity assessment and phytochemical studies of Clerodendrum thomsoniae Balf. f. with special reference to its anti-stress properties 毛松的显微繁殖、遗传保真度评价及植物化学研究。F.特别注意其抗应力性能
Pub Date : 2019-03-12 DOI: 10.25081/RIPB.2019.V9.3779
Pallab Kar, A. Chakraborty, M. Bhattacharya, T. Mishra, Arnab Sen
Clerodendrum thomsoniae commonly known as bleeding heart vine or bag flower which is a good candidate for a new crop for the floriculture industry. In this study, in-vitro callus regeneration of C. thomsoniae through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 2 mg/l BAP and 0.5 mg/l
汤姆松(Clerodendrum thomsoniae)俗称血心藤或袋花,这是一个很好的候选新作物为花卉产业。在本研究中,我们尝试了通过结培养的方法在体外培养thomsoniae愈伤组织。Murashige和Skoog培养基中添加2mg /l BAP和0.5 mg/l
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引用次数: 9
GENETIC VARIABILITY AND INTERRELATIONSHIP STUDIES IN GREEN SUPER RICE 绿色超级稻遗传变异及亲缘关系研究
Pub Date : 2018-10-13 DOI: 10.25081/ripb.2018.v8.3705
Touheed Iqbal
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引用次数: 1
期刊
Research in Plant Biology
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