尸体器官的DNA:两种提取方法的比较。

Beitrage zur gerichtlichen Medizin Pub Date : 1992-01-01

G Kinzinger, J Holtz

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引用次数: 0

摘要

采用两种不同的方法从组织标本中分离基因组DNA。与苯酚/氯仿法相比，根据Higuchi法快速提取的DNA产品收率更高，质量更好。根据DNA的数量和质量进行比较，发现脑组织的回收率最高，其次是肺和肝脏。DNA的稳定性直接受到死后年龄和身体年龄的影响。

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[DNA of cadaver organs: comparison of two methods of extraction].

Genomic DNA was isolated from tissue specimen by two different methods. The rapid extraction according to Higuchi provided increased product yield of DNA in a better quality than the extraction with phenol/chloroform. Comparing the organ tissues according to quantity and quality of DNA it was found that best yield was recovered from brain tissue followed by lung and liver. DNA stability is directly influenced by postmortal age and age of the bodies.

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Beitrage zur gerichtlichen Medizin

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