不同提取溶剂对青叶抗氧化和抗炎活性影响的体外研究。

B. Oso, N. O. Abey, O. Oyeleke, B. Olowookere
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引用次数: 0

摘要

摘要本文研究了不同提取溶剂对平叶苔藓干叶提取物酚类物质含量、抗氧化活性和抗炎活性的影响。分析采用分光光度法,用福林-乔卡多试剂估计总酚含量。通过铁还原力(FRP)和1,1-二苯基-2-苦酰肼(DPPH)自由基清除试验来评估水、乙醇、乙酸乙酯和正己烷提取物的抗氧化活性,通过热处理白蛋白的稳定性和蛋白酶的抑制作用来评估提取物的抗炎潜力。结果表明,溶剂性质对提取物的TPC含量、抗氧化和抗炎活性有显著影响。水提液TPC最高。在水提液和乙醇提液中,玻璃钢同样高。乙醇提取物具有最高的自由基清除活性和抗炎活性。结果表明,水和乙醇可作为提取风干叶中活性物质的合适溶剂-à-vis抗氧化和抗炎活性物质。
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In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam.
Summary This study investigated the effect of different extraction solvents on the phenolic contents (TPC), antioxidant activities, and anti-inflammatory potentials of dried-leaf extracts of Bryophyllum pinnatum Lam. The analyses were performed spectrophotometrically, using a Folin-Ciocalteau reagent for the estimation of the total phenolic contents. The ferric reducing power (FRP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays were carried out to assess the antioxidant activities of aqueous, ethanolic, ethyl acetate, and n-hexane extracts of the plant, whereas the stabilization of heat-treated albumin and inhibition of protease action were performed to evaluate the anti-inflammatory potentials of the extracts. The results obtained indicate that the solvent nature significantly influenced the amount of TPC, antioxidant and anti-inflammatory potentials of the extracts. The aqueous extract had the highest TPC. The FRP was equally high in the aqueous and ethanol extracts. The ethanolic extract had the highest radical scavenging activity and anti-inflammatory potentials. The results show that water and ethanol could be suitable solvents for the extraction of bioactive compounds from the dried leaf of B. pinnatum vis-à-vis antioxidant and anti-inflammatory potentials.
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