Preparation of Centella asiatica (L). and Hypericum perforatum (St. John's Wort) Plant Extracts and Development of Anti-Aging Herbal Cream Formulations

Objective: The aim of the study is to use two different plant extracts such as St. John's Wort (Hypericum perforatum) and Centella asiatica (L.) in the cream formulation and to determine the anti-aging effect of the new formulation. Material-Method: The plants used in the study were obtained commercially and plant extracts were obtained using the classical extraction techniques in the literature. The active ingredients in the obtained extracts were determined by HPLC method. Physical, protective efficacy, microbial analysis and anti-aging tests were carried out for cream formulations obtained with extracts. Results: Plant extraction studies were carried out in the study. Centella asiatica (L.) was extracted with 20.8% yield at 20 mL of methanol and 60 ℃. As a result of HPLC analysis, it was determined that there were 1740 mg/kg asiatic acid and 4380 mg/kg madecasic acid in the extract. In the extraction studies performed on Hypericum perforatum, the expected active ingredient, hypericin, was not found. For this reason, commercial extract containing 2.5% hyperforin was obtained and the studies were continued on these extracts and final cream formulations were obtained. Conclusion: pH, viscosity, density, protective effectiveness tests and microbial analysis tests of the final formulations were performed. In addition, in vitro anti-aging studies have been carried out in accredited laboratories. With in-vitro anti-aging tests, it was determined that the level of collagen 1A increased more in the formulation where both extracts were used together.