采用Uv-Vis光谱学方法对类黄酮和水生菜乙醇乙醇提取物进行检测

Dewi Rahmayani Rahmayani
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摘要

Selada air(旱金莲Officinale R.Br)广泛存在于印度尼西亚,具有抗氧化剂的潜力。本研究的目的是分析塞拉达空气提取物的黄酮含量和抗氧化活性。以70%乙醇为溶剂,采用浸渍法提取塞拉达空气。采用紫外-可见分光光度法测定提取物的类黄酮含量,DPPH法测定提取物的抗氧化活性。本研究结果表明,该化合物的黄酮含量为13.9 mgQE/g提取物,抗氧化活性IC50值为102,26 ppm,属中等抗氧化范畴。关键词:抗氧化剂;DPPH,黄酮类化合物:旱金莲
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Uji Kadar Senyawa Flavonoid Dan Antioksidan Ekstrak Etanol Selada Air (Nasturtium Officinale R.Br) Menggunakan Metode Spektrofotometri Uv-Vis
Selada air (Nasturtium Officinale R.Br) are widely found in Indonesia and have potential as antioxidants. The purpose of this study was to analyze the levels of flavonoids and antioxidant activity of Selada air extract. Selada air were extracted by maceration method with 70% ethanol as solvent. The flavonoid content of the extract was measured by the UV-Vis Spectrophotometry method, while the antioxidant activity of the extract was determined by the DPPH method. The results of this study indicate that the ethanol extract of the Selad air has a flavonoid content of 13,9 mgQE/g extract and the antioxidant activity of the compound obtained an IC50 value of 102,26 ppm with a moderate antioxidant category. Keywords: Antioxidant; DPPH, Flavonoids: Nasturtutium Officinale R.Br
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