一氧化氮供体纳米包封对凤梨科花蕾萌发的影响

Kauê Alexandre Monteiro, Jean Carlo Baudraz Paula, Gabriel Danilo Shimizu, Walter Aparecido Ribeiro Júnior, Hugo Roldi Guariz, Ricardo Tadeu Faria
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摘要

凤梨花因其叶子和花朵的美丽而被用于景观美化。利用植物调节剂如一氧化氮(NO)促进萌发,是提高产量和减少对自然界植物的寻找的一条出路,纳米胶囊化的目的是优化其效果。研究了游离一氧化氮供体和纳米包封一氧化氮供体对紫苏萌发的影响。分别用15 mM和20 mM剂量的s-亚硝基谷胱甘肽(GSNO)、含GSNO的壳聚糖/三聚磷酸钠纳米颗粒(NP CS/TPP-GSNO)和空壳聚糖/TPP浸泡种子5 min,对照采用蒸馏水渗吸。每处理4个重复,每处理50粒种子。除幼苗长度(SL)和幼苗干重(SDW)外,还对发芽百分率(GER)、首次发芽计数(FGC)、发芽速度指数(GSI)、平均发芽时间(t)进行了评价。为了对种子进行表征,对其含水量和活力进行了评价。种子含水量为9.9%,活力为64%。对于GER, GSNO的治疗范围从43%到60%不等。GSNO的施用刺激了金丝桃的萌发过程,纳米包封与游离GSNO相比,结果没有任何差异。结论是,该应用程序
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Influence of nitric oxide donor nanoencapsulation on Dyckia excelsa Leme (Bromeliaceae) germination
Bromeliads are used in landscaping due to the beauty of their leaves and flowers. The use of plant regulators such as nitric oxide (NO) promotes the stimulus of germination and it has been a way out to enhance production and reduce the search for plants in nature and nanoencapsulation aims to optimize its effect. The objective of this work was to evaluate the effect of using free and nanoencapsulated NO donor on the germination of D. excelsa. The treatments consisted of soaking the seeds for 5 minutes with s-nitrosoglutathione (GSNO), chitosan/sodium tripolyphosphate nanoparticles containing GSNO (NP CS/TPP-GSNO) and empty (NP CS/TPP) at doses: 15 mM and 20 mM. The control consisted of imbibition in distilled water. For each treatment, 4 replications of 50 seeds were used. The following variables were evaluated: percentage of germination (GER), first germination count (FGC), germination speed index (GSI), mean germination time (t) in addition to the length (SL) and seedling dry weight (SDW). To characterize the seeds, water content and viability were evaluated. D. excelsa seeds had 9.9% water content and 64% viability. For GER, treatments with GSNO ranged from 43 to 60%. The application of GSNO stimulated the germination process of D. excelsa and the nanoencapsulation did not cause any difference in the results compared to the free GSNO. It was concluded that the application
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