从尼拉鱼(Oreochromis niloticus)消化道(Oreochromis)中分离出一种乳酸(BAL)细菌,以及它抑制菌根球菌和白喉菌sp的能力。

A. Bukhori, Sartini Sartini, Rahmiati Rahmiati
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引用次数: 5

摘要

乳酸菌具有能够发酵糖或碳水化合物并产生乳酸作为发酵的最终产物的特性。本研究的目的是从罗非鱼消化道中分离乳酸菌,研究乳酸菌对金黄色葡萄球菌和志贺氏菌生长的抑制能力。乳酸菌采用MRSA培养基进行分离,乳酸菌分离后采用碟扩散法观察对金黄色葡萄球菌和志贺氏菌生长的抑制能力。然后对分离得到的菌株进行形态和生化表征。从研究中得到了sp1和sp2两种乳酸菌。乳酸菌sp1和sp2对金黄色葡萄球菌和志贺菌sp均有抑制作用,其中sp2对金黄色葡萄球菌的抑制作用最大,为8.75 mm,对志贺菌sp的抑制作用最大,为7.16 mm。根据本研究的分离鉴定结果,罗非鱼消化道乳酸菌sp1和sp2能够抑制金黄色葡萄球菌(数量为8.75 mm)和志贺氏菌(数量为7.16 mm)。
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Isolasi Bakteri Asam Laktat (BAL) dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) dan Kemampuannya Dalam Menghambat Staphylococcus aureus dan Shigella sp.
Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.
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