基于PPARG基因SNPS分析的鸡遗传品种遗传变异性

T. Larkina, A. Krutikova, G. Peglivanyan, N. V. Dementiev
{"title":"基于PPARG基因SNPS分析的鸡遗传品种遗传变异性","authors":"T. Larkina, A. Krutikova, G. Peglivanyan, N. V. Dementiev","doi":"10.52419/issn2072-2419.2021.4.97","DOIUrl":null,"url":null,"abstract":"Obesity of chickens significantly reduces the efficiency of feeding, egg production, palatability of meat carcasses, slaughter yield, consumer value, and there is also such an ecological aspect as the problem of fat utilization. The search for SNPs in genes involved in lipid metabolism is an important task today for modern research in the field of poultry farming. Differences in carcass fat between breeds and lines within a breed illustrate the importance of genetic factors in lipid metabolism in chickens. The aim of the study was to find and analyze SNPs in the PPARG gene by sequencing in various chicken breeds (n = 83). The objects of the study were chickens from the VNIIGZh bioresource collection \"Genetic collection of rare and endangered chicken breeds\" (Pushkin, St. Petersburg) of various productivity trends (n = 83). Blood for DNA isolation was taken from hens at the age of 330 days from a wing vein into a microtube containing 50 μl of 0.5 mM EDTA as an anticoagulant. Prior to use, blood samples were stored at -200C. Genomic DNA was isolated using a standard phenolic-detergent method. The concentration and purity of the samples were determined using a NanoDrop 2000 instrument (Thermo Fisher, USA).Analysis of the polymorphism of the regulatory region of the PPARG gene was carried out by sequencing. The primer design was carried out on the basis of information from the Internet database.The studies were carried out on the basis of the laboratory of molecular genetics of the RRIFAGB. Genetic variability for rs314476701 of the PPARG gene was determined in the analyzed breeds. SNP rs316237745 revealed a strong shift of the A.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"14 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic variability of genetic chicken breeds estimated based on SNPS analysis in the PPARG gene\",\"authors\":\"T. Larkina, A. Krutikova, G. Peglivanyan, N. V. Dementiev\",\"doi\":\"10.52419/issn2072-2419.2021.4.97\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Obesity of chickens significantly reduces the efficiency of feeding, egg production, palatability of meat carcasses, slaughter yield, consumer value, and there is also such an ecological aspect as the problem of fat utilization. The search for SNPs in genes involved in lipid metabolism is an important task today for modern research in the field of poultry farming. Differences in carcass fat between breeds and lines within a breed illustrate the importance of genetic factors in lipid metabolism in chickens. The aim of the study was to find and analyze SNPs in the PPARG gene by sequencing in various chicken breeds (n = 83). The objects of the study were chickens from the VNIIGZh bioresource collection \\\"Genetic collection of rare and endangered chicken breeds\\\" (Pushkin, St. Petersburg) of various productivity trends (n = 83). Blood for DNA isolation was taken from hens at the age of 330 days from a wing vein into a microtube containing 50 μl of 0.5 mM EDTA as an anticoagulant. Prior to use, blood samples were stored at -200C. Genomic DNA was isolated using a standard phenolic-detergent method. The concentration and purity of the samples were determined using a NanoDrop 2000 instrument (Thermo Fisher, USA).Analysis of the polymorphism of the regulatory region of the PPARG gene was carried out by sequencing. The primer design was carried out on the basis of information from the Internet database.The studies were carried out on the basis of the laboratory of molecular genetics of the RRIFAGB. Genetic variability for rs314476701 of the PPARG gene was determined in the analyzed breeds. SNP rs316237745 revealed a strong shift of the A.\",\"PeriodicalId\":378052,\"journal\":{\"name\":\"International bulletin of Veterinary Medicine\",\"volume\":\"14 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International bulletin of Veterinary Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.52419/issn2072-2419.2021.4.97\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International bulletin of Veterinary Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52419/issn2072-2419.2021.4.97","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

鸡的肥胖显著降低了饲养效率、产蛋率、胴体的适口性、屠宰产量、消费价值,还存在着脂肪利用等生态方面的问题。寻找与脂质代谢有关的基因的snp是当今家禽养殖领域现代研究的一项重要任务。不同品种和品种间胴体脂肪的差异说明了遗传因素在鸡脂质代谢中的重要性。本研究的目的是通过测序在各种鸡品种(n = 83)中发现和分析PPARG基因的snp。研究对象为VNIIGZh生物资源库“珍稀和濒危鸡种遗传集”(Pushkin, St. Petersburg)中各种生产力趋势的鸡(n = 83)。取330日龄鸡翅膀静脉的血液,放入含有50 μl 0.5 mM EDTA抗凝剂的微管中进行DNA分离。使用前,血样保存在-200℃。基因组DNA采用标准的酚醛洗涤剂方法分离。样品的浓度和纯度使用NanoDrop 2000仪器(Thermo Fisher, USA)测定。通过测序对PPARG基因调控区进行多态性分析。引子设计是根据Internet数据库的信息进行的。研究是在RRIFAGB分子遗传学实验室的基础上进行的。测定了PPARG基因rs314476701的遗传变异性。SNP rs316237745显示了a的强烈移位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Genetic variability of genetic chicken breeds estimated based on SNPS analysis in the PPARG gene
Obesity of chickens significantly reduces the efficiency of feeding, egg production, palatability of meat carcasses, slaughter yield, consumer value, and there is also such an ecological aspect as the problem of fat utilization. The search for SNPs in genes involved in lipid metabolism is an important task today for modern research in the field of poultry farming. Differences in carcass fat between breeds and lines within a breed illustrate the importance of genetic factors in lipid metabolism in chickens. The aim of the study was to find and analyze SNPs in the PPARG gene by sequencing in various chicken breeds (n = 83). The objects of the study were chickens from the VNIIGZh bioresource collection "Genetic collection of rare and endangered chicken breeds" (Pushkin, St. Petersburg) of various productivity trends (n = 83). Blood for DNA isolation was taken from hens at the age of 330 days from a wing vein into a microtube containing 50 μl of 0.5 mM EDTA as an anticoagulant. Prior to use, blood samples were stored at -200C. Genomic DNA was isolated using a standard phenolic-detergent method. The concentration and purity of the samples were determined using a NanoDrop 2000 instrument (Thermo Fisher, USA).Analysis of the polymorphism of the regulatory region of the PPARG gene was carried out by sequencing. The primer design was carried out on the basis of information from the Internet database.The studies were carried out on the basis of the laboratory of molecular genetics of the RRIFAGB. Genetic variability for rs314476701 of the PPARG gene was determined in the analyzed breeds. SNP rs316237745 revealed a strong shift of the A.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Correction of the functional state of the regulatory systems of the body of dogs under the influence of environmental stress factors About the conference dedicated to the 100th anniversary of the department of pathological physiology St. Petersburg state university of veterinary medicine Comparative characteristics of local anesthetics in brachial plexus block for surgery in dogs Oxidative stress in suyagny sheep at the end of gestation, as a factor in pathogenesis development of eclampsia Subclinical ketosis as a factor reduction of reproductive indicators of high productive cows
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1